Plasmids   Select Another Resource Report Type

http://www.addgene.org/54391

Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.

Proper citation: RRID:Addgene_54391 Copy   

•   Source: Addgene

http://www.addgene.org/91570

Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:26060277
Comments: Mariner himar1C9 transposase driven by lac promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Chloramphenicol resistance is contained on the plasmid backbone. For further background, see: Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017. Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.

Proper citation: RRID:Addgene_91570 Copy   

•   Source: Addgene

http://www.addgene.org/59947

Species: Synthetic
Genetic Insert: Splitposon w/ KanR, LacI & PTac
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25080493

Proper citation: RRID:Addgene_59947 Copy   

•   Source: Addgene

http://www.addgene.org/65220

Species: Pseudomonas putida strain CBB5
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23654268
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3. Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100). N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See: Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049. For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC). Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_65220 Copy   

•   Source: Addgene

http://www.addgene.org/69771

Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:27084942
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.

Proper citation: RRID:Addgene_69771 Copy   

•   Source: Addgene

http://www.addgene.org/51778

Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477

Proper citation: RRID:Addgene_51778 Copy   

•   Source: Addgene

http://www.addgene.org/51775

Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477

Proper citation: RRID:Addgene_51775 Copy   

•   Source: Addgene

http://www.addgene.org/51774

Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477

Proper citation: RRID:Addgene_51774 Copy   

•   Source: Addgene

http://www.addgene.org/222070

Vector Backbone Description: Vector Backbone:pVP077; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:39292669

Proper citation: RRID:Addgene_222070 Copy   

•   Source: Addgene

http://www.addgene.org/222072

Vector Backbone Description: Vector Backbone:pVP077; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:39292669

Proper citation: RRID:Addgene_222072 Copy   

•   Source: Addgene

http://www.addgene.org/222071

Vector Backbone Description: Vector Backbone:pVP077; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:39292669

Proper citation: RRID:Addgene_222071 Copy   

•   Source: Addgene

http://www.addgene.org/222073

Vector Backbone Description: Vector Backbone:pVP077; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:39292669

Proper citation: RRID:Addgene_222073 Copy   

•   Source: Addgene

http://www.addgene.org/231307

Species: Synthetic
Genetic Insert: BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
Vector Backbone Description: Backbone Marker:New England Biolabs; Vector Backbone:pGGAselect; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:39919271

Proper citation: RRID:Addgene_231307 Copy   

•   Source: Addgene

http://www.addgene.org/236186

Species: Synthetic
Genetic Insert: Kanamycin resistance cassette
Vector Backbone Description: Backbone Size:2824; Vector Backbone:pBTK1001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:40827214

Proper citation: RRID:Addgene_236186 Copy   

•   Source: Addgene

http://www.addgene.org/246715

Species: Arabidopsis thaliana
Genetic Insert: Basal stem of Arabidopsis MIR390a precursor with A18G mutation and a chloramphenicol-ccdB cassette flanked by 2 BsaI sites
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:41505763

Proper citation: RRID:Addgene_246715 Copy   

•   Source: Addgene

http://www.addgene.org/218891

Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist Kan High Copy; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:38986614
Comments: Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.

Proper citation: RRID:Addgene_218891 Copy   

•   Source: Addgene

http://www.addgene.org/218892

Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:pTwist Kan High Copy; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:38986614
Comments: Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.

Proper citation: RRID:Addgene_218892 Copy   

•   Source: Addgene

http://www.addgene.org/255046

Species: Syrian Golden Hamster
Genetic Insert: full length hamster prion protein (aa 23-231)
Vector Backbone Description: Vector Backbone:pET-41a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:21152012
Comments: DNA Sequences coding for hamster (residues 23-231; accession K02234) rPrPc residues were amplified and ligated into the pET41 vector (EMD Biosciences) and sequences were verified. Plasmids were transformed into E. coli Rosetta cells (EMD Biosciences).

Proper citation: RRID:Addgene_255046 Copy   

•   Source: Addgene

http://www.addgene.org/227965

Species: Other
Genetic Insert: B/c
Vector Backbone Description: Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:40105245
Comments: Please visit https://doi.org/10.1101/2024.12.18.629176 for bioRxiv preprint.

Proper citation: RRID:Addgene_227965 Copy   

•   Source: Addgene

http://www.addgene.org/227962

Species: Other
Genetic Insert: B/c
Vector Backbone Description: Backbone Size:2581; Vector Backbone:pENTR; Vector Types:; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:40105245
Comments: Please visit https://doi.org/10.1101/2024.12.18.629176 for bioRxiv preprint.

Proper citation: RRID:Addgene_227962 Copy   

•   Source: Addgene