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Species: Synthetic gene
Genetic Insert: Turbo
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24658 Copy
Species: Synthetis gene
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24657 Copy
Genetic Insert: pKW08-Lx
Vector Backbone Description: Backbone Size:7960; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25012 Copy
Genetic Insert: int L5; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This expression vector integrates at the mycobacteriophage L5 site and can be stabilized by excision of the vector backbone including the int gene using the Cre recombinase; contains xylE gene as a reporter for integration; terminators to protect expression cassette against transcriptional interference.
Proper citation: RRID:Addgene_32376 Copy
Genetic Insert: gfpm2+
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Mycobacterial GFP expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18439872
Comments: This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).
Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554
Proper citation: RRID:Addgene_32362 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Wasabi. The Wasabi ORF is present at bp# 39-749.
Proper citation: RRID:Addgene_30174 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Teal. The Teal ORF is present at bp# 103-813.
Proper citation: RRID:Addgene_30176 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Crimson. The Crimson ORF is present at bp# 104-781.
Proper citation: RRID:Addgene_30178 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with EBFP2. The EBFP2 ORF is present at bp# 104-823.
Proper citation: RRID:Addgene_30177 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Addgene Plasmid 30171; Backbone Size:0; Vector Backbone:pMSP12::dsRed2; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Comments: This plasmid was derived from pMSP12::dsRed2 by interupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.
Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#4947 when compared to the full sequence information provided by the depositing laboratory. This mismatch is not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_30170 Copy
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5304; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
This is a control plasmid for pSC301 in which the SOD promoter was excised.
Proper citation: RRID:Addgene_31852 Copy
Genetic Insert: n/a
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pSMT3; Vector Types:Shuttle vector for mycobacteria; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Comments: Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb) 87: 481–488.
There are known discrepancies between the Addgene QC sequence and the depositor's full sequence--these are likely due to how the full sequence was assembled and should not affect function.
Proper citation: RRID:Addgene_26589 Copy
Species: synthetic gene
Genetic Insert: P#38-luxAB-ssrAec
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27062 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp-ssrAec
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27059 Copy
Species: synthetic gene
Genetic Insert: P#38-gfp-ssrAec
Vector Backbone Description: Backbone Size:5574; Vector Backbone:pTE-0X1-GCT1; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27053 Copy
Species: synthetic gene
Genetic Insert: P#38-luxAB-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27063 Copy
Species: synthetic gene
Genetic Insert: P#38-gfp
Vector Backbone Description: Backbone Size:6055; Vector Backbone:pTE-0X1-GCT5; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP under the control of a strong promoter
Proper citation: RRID:Addgene_27051 Copy
Genetic Insert: tetR(sB), clpXtb
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_27373 Copy
Species: Homo sapiens
Genetic Insert: PIK3C3 and PIK3R4
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6321; Vector Backbone:pVitro2; Vector Types:Mammalian Expression, bicistronic; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18957027
Proper citation: RRID:Addgene_24055 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119884 Copy
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