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Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, R163A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2RR R163A
Proper citation: RRID:Addgene_40152 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, K162A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2RR K162A
Proper citation: RRID:Addgene_40151 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (C56S)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR C56S
Proper citation: RRID:Addgene_40146 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R183-5A + 7SA)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R183-5A + 7SA
7SA mutations are: S190A, S197A, S241A, S265A, S295A, S301A, and S335A
Note: A deletion of amino acid residues 502-523 near the C-terminus of the protein was discovered during the Addgene quality control process and is present in this plasmid.
Proper citation: RRID:Addgene_40141 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa244-468)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 aa245-STOP (RNAi res) +3’UTR
Proper citation: RRID:Addgene_40121 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa1-244) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 aa1-245 (RNAi res) +3’UTR
Proper citation: RRID:Addgene_40120 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa 345 - 468)
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004).
Alternate plasmid name: pIC26 Dendra + MEX-5 (aa345-STOP) (Dendra + MEX-5 ORF)
Proper citation: RRID:Addgene_40086 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164892 Copy
Species: Caenorhabditis elegans
Genetic Insert: YFP
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164893 Copy
Species: Caenorhabditis elegans
Genetic Insert: nCre
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164895 Copy
Species: Caenorhabditis elegans
Genetic Insert: rpl28-rtTA(Q)
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164886 Copy
Species: Caenorhabditis elegans
Genetic Insert: rtetR-zip
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164889 Copy
Species: Caenorhabditis elegans
Genetic Insert: QFAD mutated
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30634168
Proper citation: RRID:Addgene_164887 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in body wall muscle. Inject at 5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW79 has 10 random mutations relative to the pCFJ104 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177339 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in pharyngeal muscle. Inject at 2.5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW77 has 11 random mutations relative to the pCFJ90 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177338 Copy
Species: Caenorhabditis elegans
Genetic Insert: grh-1
Vector Backbone Description: Vector Backbone:pdestMB14 (Addgene #26415); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35013237
Proper citation: RRID:Addgene_177762 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16a
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6000; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_175283 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-16a mini-gene
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSL1180; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_175284 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166976 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166973 Copy
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