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Species: Xenopus laevis
Genetic Insert: TRPS1 deltaN mut
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11036 Copy
Species: Mus musculus
Genetic Insert: TRPS1 P1
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:xenopus, mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: Please see Momeni et al (Nature Genetics 24: 71-74) to learn more about the patient mutation.
Proper citation: RRID:Addgene_11035 Copy
Species: Homo sapiens
Genetic Insert: Neurogenin 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6000; Vector Backbone:pUCM; Vector Types:Mammalian Expression, Cre/Lox, CRISPR, TALEN; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110492 Copy
Species: Homo sapiens
Genetic Insert: Yes
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_110497 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#26655; Backbone Size:6783; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110410 Copy
Species: Pontellina plumata
Genetic Insert: turboGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO/FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110375 Copy
Species: Homo sapiens
Genetic Insert: ELAVL1 (HuR)
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110411 Copy
Species: Homo sapiens
Genetic Insert: ELAVL1 (HuR)
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110412 Copy
Species: Homo sapiens
Genetic Insert: HuR (ELAVL1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3.1D/V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110417 Copy
Species: Homo sapiens
Genetic Insert: HRPT2 136X
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Author has deposited insert sequence. Click on "sequence" to view. Cloned using the following primers 5' CGGGATCCATGGCGGACGTGCTTAGCGT 3' and 5' CCGCTCGAGCTATGCTTCTGCTAAAACTTC 3' and ligated into pcDNA3 HRPT2 that had been digested with BamHI & XhoI (which removes full-length HRPT2, but leaves the flag tag at the N-terminus. See plasmid 11048 to learn how pcDNA3 HRPT2 was constructed).
Proper citation: RRID:Addgene_11049 Copy
Species: Homo sapiens
Genetic Insert: HRPT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Author has deposited insert sequence. Click on "sequence" to view. Cloned using the following primers 5' CGGAATTCGGATCCACCATGGATTACAAGGATGACGACGATAAGGTCGACATGGCGGACGTGCTTAGCGT 3' and 5' CCGCTCGAGTCAGAATCTCAAGTGCGATTTATGCTT 3'
Proper citation: RRID:Addgene_11048 Copy
Species: Xenopus laevis
Genetic Insert: TRPS1 delN delC119 mut
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:xenopus, mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: Can be expressed in cos cells, gives the correct sized product by in-vitro tranlation and can be expressed in xenopus embryos.
Proper citation: RRID:Addgene_11045 Copy
Species: Xenopus laevis
Genetic Insert: TRPS1 mut
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: Can be expressed in cos cells, does not give correct sized product by in-vitro translation, and cannot be expressed in xenopus embryos.
Proper citation: RRID:Addgene_11047 Copy
Species: Homo sapiens
Genetic Insert: SOX4
Vector Backbone Description: Backbone Marker:William Sellers; Vector Backbone:pcDNA3-Flag-FKHR; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110360 Copy
Species: Xenopus laevis
Genetic Insert: TRPS1
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11046 Copy
Species: Homo sapiens
Genetic Insert: HOXC6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3-HISA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110365 Copy
Species: Xenopus laevis
Genetic Insert: TRPS1
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11041 Copy
Species: Homo sapiens
Genetic Insert: HOXC6
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3-HISA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110366 Copy
Species: Mus musculus
Genetic Insert: TRPS1 mut
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:xenopus, mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: Full coding + UTR of mTRPS1. Can be expressed in cos cell, xenopus embryos, and in vitro translation.
Proper citation: RRID:Addgene_11040 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:#42230; Backbone Size:8506; Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: pX330 with puromycin resistance
For plasmid usage, please see the associated publication (Cong et al. Science. 2013, PMID: 23287718), as well as Ran et al. Nat Protoc. 2013, PMID: 24157548.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
Proper citation: RRID:Addgene_110403 Copy
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