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Species: Human cytomegalovirus (CMV)
Genetic Insert: CMV UL44 (38-52)
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165021 Copy
Species: Homo sapiens
Genetic Insert: HLA-B*51:01
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303 (Addgene #25897); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165011 Copy
Species: Homo sapiens
Genetic Insert: HLA-DRA
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303 (Addgene #25897); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165012 Copy
Species: Human cytomegalovirus (CMV)
Genetic Insert: CMV US20 (29-37)
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165018 Copy
Species: Human cytomegalovirus (CMV)
Genetic Insert: CMV UL83 (363-373)
Vector Backbone Description: Backbone Size:9531; Vector Backbone:pLX301 (Addgene #25895); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165016 Copy
Species: Homo sapiens
Genetic Insert: HLA-A*24:02
Vector Backbone Description: Backbone Size:9335; Vector Backbone:pLX303 (Addgene #25897); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165010 Copy
Species:
Genetic Insert: Smad binding element
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: SBE-luc was made by annealing the oligonucleotides 5′-CGGAGTATGTCTAGACTGACAATG-3′ and 5′-CTAGCATTGTCAGTCTAGACATACTCCGAGCT-3′ and cloning them into the Sac1 and Nhe1 sites.
Proper citation: RRID:Addgene_16527 Copy
Species:
Genetic Insert: FAST-1 binding element
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: FBE-luc was made by annealing the oligonucleotides 5′-CGGATTGTGTATTGGCTGTAC-3′ and 5′-AGCCAATACACAATCCGGTAC-3′ and cloning them into the KpnI site.
Proper citation: RRID:Addgene_16526 Copy
Species: Homo sapiens
Genetic Insert: FAST-1 H83R
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16522 Copy
Species: Homo sapiens
Genetic Insert: FAST-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: To construct an hFAST-1 expression vector, normal human colon cDNA was used as the template to PCR-amplify the hFAST-1 ORF with primers NT2-exp5′ (5′-TATGCGGCCGCCACCATGGGGCCCTGCAGCG-3′) and NT2-exp3′ (5′-TATGCGGCCGCGAGCTGCTGTGTCGCAGAC-3′). The PCR product was cloned into the NotI site of pCI-neo.
Proper citation: RRID:Addgene_16521 Copy
Species: Homo sapiens
Genetic Insert: beta catenin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: HCT116 has a 3-bp deletion that removes one amino acid (Ser45).
Proper citation: RRID:Addgene_16520 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16539 Copy
Species: Homo sapiens
Genetic Insert: mCherry-ACTB-P2A-EGFP
Vector Backbone Description: Backbone Marker:Scott Lowe; Backbone Size:5795; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165158 Copy
Species:
Genetic Insert: 4xDRE
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Contains 4 copies of a putative PPARdelta-responsive element (DRE, 5'-CGCTCACAGGTCA-3') generated by joining the PPARdelta- and RXRalpha- binding sites.
Proper citation: RRID:Addgene_16534 Copy
Species:
Genetic Insert: 4xACO
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Contains 4 copies of the prototypic PPAR-responsive element ACO (5'-AGGACAAAGGTCA-3'). The prototypic PPAR response element ACO from the acyl-CoA oxidase gene promotor contains two copies of the core binding sequence AGGTCA separated by one base pair.
Proper citation: RRID:Addgene_16533 Copy
Species: Homo sapiens
Genetic Insert: E6AP Hect
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
Proper citation: RRID:Addgene_165102 Copy
Species: Homo sapiens
Genetic Insert: E6AP isoform 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
Proper citation: RRID:Addgene_165100 Copy
Species: Homo sapiens
Genetic Insert: Nedd4.2d
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Best yields for protein purification were found in BL21 (DE) cells using an overnight induction at 16 degrees C.
Proper citation: RRID:Addgene_165103 Copy
Species: Homo sapiens
Genetic Insert: Nedd4.2d F823A
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:7013; Vector Backbone:pDEST15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Best yields for protein purification in BL21 AI cells using an overnight induction at 16 degrees C.
5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: EcoRI (not destroyed).
Proper citation: RRID:Addgene_165104 Copy
Species: SARS-COV-2
Genetic Insert: Nsp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165108 Copy
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