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Species: Synthetic
Genetic Insert: CjeCas9 sgRNA entry vector
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32218510
Proper citation: RRID:Addgene_133793 Copy
Species: Synthetic
Genetic Insert: St3Cas9 sgRNA entry vector
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32218510
Proper citation: RRID:Addgene_133792 Copy
Species: Synthetic
Genetic Insert: human codon optimized NmeCas9
Vector Backbone Description: Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32218510
Proper citation: RRID:Addgene_133790 Copy
Species: Rattus norvegicus
Genetic Insert: Syt1
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501440
Comments: 5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: EcoRI (destroyed during cloning)
Proper citation: RRID:Addgene_133825 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: p1k-COX9-mTagBFP2
Vector Backbone Description: Vector Backbone:Clhn-mTagBFP2-Trp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31481383
Proper citation: RRID:Addgene_133669 Copy
Species: Rattus norvegicus
Genetic Insert: Syt1
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501440
Comments: 5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: EcoRI (destroyed during cloning)
Proper citation: RRID:Addgene_133823 Copy
Species: Synthetic
Genetic Insert: human codon optimized CjeCas9
Vector Backbone Description: Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32218510
Proper citation: RRID:Addgene_133789 Copy
Species: Rattus norvegicus
Genetic Insert: Syt1
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501440
Comments: 5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: EcoRI (destroyed during cloning)
Proper citation: RRID:Addgene_133822 Copy
Species: Synthetic
Genetic Insert: human codon optimized St3Cas9
Vector Backbone Description: Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32218510
Proper citation: RRID:Addgene_133788 Copy
Genetic Insert: pAAV-EF1a-DIO-tdTomato-WPRE
Vector Backbone Description: Backbone Marker:karl deisseroth; Vector Backbone:pAAV-EF1a-double floxed-TdTomato-WPRE; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31591211
Comments: Please note: Plasmid contains a T230I mutation in tdTomato. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_133786 Copy
Species: Rattus norvegicus
Genetic Insert: PSD95-EGFP
Vector Backbone Description: Backbone Marker:karl deisseroth; Vector Backbone:pAAV-EF1a-double floxed-TdTomato-WPRE; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31591211
Comments: Please note: Plasmid contains FS793-794LT, A892V, and H932T mutations in the insert sequence, which all fall within EGFP. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_133785 Copy
Vector Backbone Description: Backbone Size:4465; Vector Backbone:pUC57; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32603702
Proper citation: RRID:Addgene_133784 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30051818
Comments: HMS clone ID number: EvNO00483431.
Proper citation: RRID:Addgene_133783 Copy
Species: Other
Genetic Insert: Drosophila-optimized Cas9
Vector Backbone Description: Vector Backbone:pMK33; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30051818
Comments: HMS clone ID number: EvNO00483429.
Proper citation: RRID:Addgene_133781 Copy
Species: Rattus norvegicus
Genetic Insert: Syt1
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501440
Comments: 5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: EcoRI (destroyed during cloning)
Proper citation: RRID:Addgene_133816 Copy
Species: Other
Genetic Insert: sfGFP(SPS6)
Vector Backbone Description: Backbone Marker:Frenzel etal. 2018; Vector Backbone:pNW-Ppta-3TER; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133814 Copy
Species: Synthetic
Genetic Insert: cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
Vector Backbone Description: Vector Backbone:pAYCU268; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30867327
Comments: The plasmid contains the following relevant inserts: 1. C. albicans Autonomously Replicating Sequence 2 for episomal propagation in C. tropicalis; 2. CAS9 sequence expressed from a Meyerozyma guilliermondii promoter; 3. Cassette for the expression of the sgRNA from a Ashbya gossypii RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.
Proper citation: RRID:Addgene_133813 Copy
Species: Homo sapiens
Genetic Insert: Isocitrate dehydrogenase 1
Vector Backbone Description: Backbone Size:4828; Vector Backbone:pKT; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_133779 Copy
Genetic Insert: RhaR
Vector Backbone Description: Backbone Marker:Dongru Qiu, Hongwei D. Yu; Backbone Size:5216; Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33077967
Comments: Please note: Plasmid contains a K244R mutation in pRO1600 Rep. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_133773 Copy
Species: Homo sapiens
Genetic Insert: SUMO 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA 3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15897463
Proper citation: RRID:Addgene_133772 Copy
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