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Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149187 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724393.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149220 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725305.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149189 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site. NGS used for QC was performed by Ginkgo and may not fully cover the entire plasmid.
Proper citation: RRID:Addgene_149223 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flpe-ERT2 fusion protein
Vector Backbone Description: Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation.
Proper citation: RRID:Addgene_14756 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153106 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153101 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153105 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast transporters
Vector Backbone Description: Backbone Marker:Jean-Marc Daran; Vector Backbone:Addgene #107916: pMEL10; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33465478
Proper citation: RRID:Addgene_153104 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: α Galactosidase SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153504 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Invertase 2 SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153493 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Prepro SP
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33980981
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.07.22.216432v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HO endonuclease
Vector Backbone Description: Backbone Marker:Addgene plasmid # 64536; Backbone Size:4434; Vector Backbone:pRG206MX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33053188
Comments: Linearize plasmid with AscI for integration into the yeast genome. See Gnuegge et al., 2016 (https://pubmed.ncbi.nlm.nih.gov/26647923/) for details.
Proper citation: RRID:Addgene_154821 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Orp1
Vector Backbone Description: Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_155042 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Size:6267; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18685 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1 153-405
Vector Backbone Description: Backbone Size:6267; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18689 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sir4
Vector Backbone Description: Backbone Size:7167; Vector Backbone:pOBD2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18802438
Proper citation: RRID:Addgene_18795 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1 L94A
Vector Backbone Description: Backbone Size:6268; Vector Backbone:pRS416 Gal; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18480252
Proper citation: RRID:Addgene_18692 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste7
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pYBS311; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8062390
Comments: Lex-Ste7C has the 1 kb BglII-BamHI fragment of pYEE129 at BamHI of pYBS311
Proper citation: RRID:Addgene_18886 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Yeast ubiquitin conjugating enzyme Mms2
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pET16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15917625
Comments: Hofmann R.M. Pickart C.M. 1999, Cell 96, 645-653
Proper citation: RRID:Addgene_18893 Copy
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