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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110235 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110236 Copy
Species: Rattus norvegicus
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: cDNA encoded from 659-4371 (see author's map).
Proper citation: RRID:Addgene_11027 Copy
Species: Mus musculus
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: No stop codon.
Proper citation: RRID:Addgene_11026 Copy
Species: END-1 is from C. Elegans, EnR is from Drophila
Genetic Insert: End1::EnR
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:xenopus, mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11029 Copy
Species: Homo sapiens
Genetic Insert: IRS-1
Vector Backbone Description: Backbone Marker:available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11025 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pGEM3Z; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results detected a P127S variant in Ura4. This variant does not affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_110227 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: ura4+
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results detected a P127S variant in Ura4. This variant does not affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_110224 Copy
Species: Homo sapiens
Genetic Insert: KGA glutaminase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA3.1D/V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110332 Copy
Species: Homo sapiens
Genetic Insert: B3GNT5
Vector Backbone Description: Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110325 Copy
Species: Homo sapiens
Genetic Insert: HRASLS
Vector Backbone Description: Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110324 Copy
Species: Homo sapiens
Genetic Insert: KGA glutaminase
Vector Backbone Description: Backbone Marker:#110344; Backbone Size:7423; Vector Backbone:pQC MCS IRES G418; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: KGA.wt obtained with V5-6xHis from pcDNA3.1D V5-His-TOPO-KGA.wt (Addgene #110332)
pQC-based γ-Retroviral transfer vector for KGA.wt-V5 expression. IRES-driven Puromycin selection.
Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110390 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:#110341; Backbone Size:7757; Vector Backbone:pQC V5 mKO2 MCS IRES Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: pQC-based γ-Retroviral transfer vector for V5-mKO2-HuR.wt expression. IRES-driven Puromycin selection.
Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110387 Copy
Species: Homo sapiens
Genetic Insert: GLS glutaminase
Vector Backbone Description: Backbone Marker:#26655; Backbone Size:6783; Vector Backbone:pLKO.1-blast; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110420 Copy
Species: Homo sapiens
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:#21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110421 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:#110342; Backbone Size:7064; Vector Backbone:pQC V5 MCS IRES Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: pQC-based γ-Retroviral transfer vector for V5-HuR.wt expression. IRES-driven Puromycin selection.
Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging.
Proper citation: RRID:Addgene_110386 Copy
Species: Homo sapiens
Genetic Insert: ELAVL1 HuR
Vector Backbone Description: Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110426 Copy
Species: Mus musculus
Genetic Insert: TRPS1 P2
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:xenopus, mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: Please see Momeni et al (Nature Genetics 24: 71-74) to learn more about the patient mutation.
Proper citation: RRID:Addgene_11037 Copy
Species: Xenopus laevis
Genetic Insert: TRPS1 deltaN
Vector Backbone Description: Backbone Size:4133; Vector Backbone:pCS105; Vector Types:mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11034 Copy
Species: Homo sapiens
Genetic Insert: ELAV like RNA binding protein 1
Vector Backbone Description: Backbone Marker:#110371; Backbone Size:5834; Vector Backbone:pcDNA5/FRT/TO/FLAG-mKO2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Insert created by joining regions before (Addgene #110395) and after (Addgene #110395) HuR HNS by HindIII restriction site
Proper citation: RRID:Addgene_110373 Copy
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