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Species: Synthetic
Genetic Insert: Nano-lantern_ATP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859502. Although they differ by only three N-terminal amino acids in the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.
Proper citation: RRID:Addgene_51992 Copy
Species: Mus musculus
Genetic Insert: Dact1(1250-1601)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52046 Copy
Species:
Genetic Insert: MAVS
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51999 Copy
Species: Simian virus 12
Genetic Insert: SA12
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC119; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51995 Copy
Species: Homo sapiens
Genetic Insert: UBR5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5887; Vector Backbone:pcDNA 6.2 N-EmGFP-DEST-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid encodes a C-terminal V5 fusion, however this is not included as UBR5 cDNA insert contains 3' STOP codon.
Addgene NGS results identified a K503R mutation within the UBR5 translation. There is no information on the functional consequence of this mutation
Proper citation: RRID:Addgene_52050 Copy
Species: Homo sapiens
Genetic Insert: Mdm2 D367A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Original plasmid from Y. Xiong, Addgene plasmid #20935.
Proper citation: RRID:Addgene_52058 Copy
Species: Mus musculus
Genetic Insert: p67 myb binding protein
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4500; Vector Backbone:pCMX-Gal4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52 Copy
Species: Methanocaldococcuc maripaludis
Genetic Insert: SepRS
Vector Backbone Description: Backbone Marker:Pharmacia; Vector Backbone:pKK223-3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid is the same as pKD-SepRS-EFSep, Addgene plasmid #34623, with the addition of the 5xtRNA-Sep insert.
Certain elements of this plasmid are covered under US patent 9,090,928 to Yale University
Proper citation: RRID:Addgene_52054 Copy
Species: Mus musculus
Genetic Insert: Neurod1
Vector Backbone Description: Backbone Size:7745; Vector Backbone:Tet-O-FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's quality control sequence shows that there is a minor change in the T2A region, EF to TR; Nd1 itself or the Puro cassette are not affected.
Proper citation: RRID:Addgene_52052 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2870; Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: To clone new genes for your proteins of interest into the pCRT7 NT vector provided, use 5’KpnI--‐3’HindIII restriction sites.
See Lutz and Bujard 1997 (PMID 9092630) for information on the tetR/pLtetO promoter system used for inducible expression in the pCRT7 NT vector.
The phosphoproteins (or sfGFP) expressed via the pCRT7 pLtetO/tetR plasmid is induced with 100 ng/mL anhydrotetracycline.
Proper citation: RRID:Addgene_52053 Copy
Species: Homo sapiens
Genetic Insert: Caspase-2 shRNA
Vector Backbone Description: Backbone Marker:Addgene plasmid #20672; Backbone Size:5900; Vector Backbone:MSCV-IRES-GFP-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52061 Copy
Species:
Genetic Insert: MAVS-M142A
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52062 Copy
Species: Homo sapiens
Genetic Insert: P4HB
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 129
Proper citation: RRID:Addgene_52069 Copy
Species: Danio rerio
Genetic Insert: actinodin 1 in situ probe
Vector Backbone Description: Backbone Marker:promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52102 Copy
Species: Other
Genetic Insert: hCas9D10A
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:5800; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_52101 Copy
Species: Homo sapiens
Genetic Insert: GGH
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 122
Proper citation: RRID:Addgene_52066 Copy
Species: Homo sapiens
Genetic Insert: Thyroid Transcription Factor 1
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: When compared to GenBank reference sequence NP_003308.1, amino acids 171-221 were deleted to create this mutant. See the insert sequence provided by the depositing laboratory for the mutant TTF-1 protein sequence.
Proper citation: RRID:Addgene_52063 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:MSCV IRES GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The FP can be removed with Nco1-Not1 digest.
The Multicloning site reads EcoR1-BglII-SnaB1-BamH1-Mfe1-Xho1. These are unique. The Nhe1 site is not unique.
Proper citation: RRID:Addgene_52109 Copy
Species: Danio rerio
Genetic Insert: actinodin 3 in situ probe
Vector Backbone Description: Backbone Marker:promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52104 Copy
Species: Danio rerio
Genetic Insert: actinodin 4 in situ probe
Vector Backbone Description: Backbone Marker:promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52105 Copy
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