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Species: Homo sapiens
Genetic Insert: Bax
Vector Backbone Description: Backbone Size:4700; Vector Backbone:C3-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10228148
Proper citation: RRID:Addgene_19741 Copy
Species: Mus musculus
Genetic Insert: oligo targeting p53 and PTEN
Vector Backbone Description: Backbone Size:7994; Vector Backbone:MSCV FLIPi; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19746 Copy
Species: Mus musculus
Genetic Insert: oligo targeting p53
Vector Backbone Description: Backbone Size:7994; Vector Backbone:MSCV FLIPi; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19748 Copy
Species: Mus musculus
Genetic Insert: oligo targeting PTEN
Vector Backbone Description: Backbone Size:7994; Vector Backbone:MSCV FLIPi; Vector Types:Mammalian Expression, Retroviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.
See FLIP vector protocols (PDF from this page) for more information.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
Proper citation: RRID:Addgene_19749 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19680 Copy
Species: Caenorhabditis elegans
Genetic Insert:
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19671 Copy
Species: Caenorhabditis elegans
Genetic Insert:
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19674 Copy
Species: Mus musculus
Genetic Insert: mouse Brn2
Vector Backbone Description: Backbone Size:4801; Vector Backbone:CAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18667607
Proper citation: RRID:Addgene_19711 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R2-R1)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19679 Copy
Species: Homo sapiens
Genetic Insert: V-ral simian leukemia viral oncogene homolog A
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17174914
Proper citation: RRID:Addgene_19717 Copy
Species: Nodamura Virus
Genetic Insert: Nodamura B2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15919892
Comments: The Nodamuravirus B2 gene is subcloned from the KpnI/ApaI fragment from pcDNA3.1+puro Nodamura B2 into pEGFP-C1 vector, making the EGFP fusion construct.
Proper citation: RRID:Addgene_19716 Copy
Species: Homo sapiens
Genetic Insert: V-ral simian leukemia viral oncogene homolog A
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17174914
Proper citation: RRID:Addgene_19719 Copy
Species: Caenorhabditis elegans
Genetic Insert: hlh-12(R25K)_genomic region
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pCR-XL-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19690 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prpl-28
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDONR221; Vector Types:Worm Expression, entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19682 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phlh-12
Vector Backbone Description: Backbone Size:0; Vector Backbone:pDONR 221; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19684 Copy
Species: Homo sapiens
Genetic Insert: V-ral simian leukemia viral oncogene homolog B
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17174914
Proper citation: RRID:Addgene_19720 Copy
Species: Caenorhabditis elegans
Genetic Insert: hlh-12(R25K)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC188; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19686 Copy
Species: Caenorhabditis elegans
Genetic Insert: hlh-12(R15K)_genomic region
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:0; Vector Backbone:pCR-XL-TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19689 Copy
Species: Homo sapiens
Genetic Insert: V-ral simian leukemia viral oncogene homolog B
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17174914
Proper citation: RRID:Addgene_19721 Copy
Species: Homo sapiens
Genetic Insert: V-ral simian leukemia viral oncogene homolog A
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17174914
Proper citation: RRID:Addgene_19723 Copy
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