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Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859492
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51971 Copy
Species: Homo sapiens
Genetic Insert: HA-Daxx
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52023 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 71
Proper citation: RRID:Addgene_52022 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM-2GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859494
Proper citation: RRID:Addgene_51979 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: GenBank accession number: JN859492
Proper citation: RRID:Addgene_51972 Copy
Species: Synthetic
Genetic Insert: Nano-lantern
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859492
The minor discrepancies between the QC and depositor sequence have no functional consequence and Addgene's QC sequence should be used for comparison.
Please note the upstream KpnI site shown on the depositor's map is not present in this plasmid.
Proper citation: RRID:Addgene_51975 Copy
Species: Homo sapiens
Genetic Insert: CTGF
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 121
Proper citation: RRID:Addgene_52028 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859498
Proper citation: RRID:Addgene_51980 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_52036 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-2G
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859496
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51982 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859495
Proper citation: RRID:Addgene_51981 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: let-7 sequence for nucleotides 2-15 is CAACCTACTACCTCA.
Proper citation: RRID:Addgene_52035 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-16
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid 1497); Backbone Size:4406; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: The D->V point mutation in DAF-16 has no functional consequence.
Proper citation: RRID:Addgene_52032 Copy
Species: Homo sapiens
Genetic Insert: FGG
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 83
Co-transfect with FGA-bio-His (Addgene 52029) and FGB (Addgene 52030) to create the fibrinogen trimer
Proper citation: RRID:Addgene_52031 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_ATP0
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN974906. Although they differ by only three N-terminal amino acidsin the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.
Proper citation: RRID:Addgene_51989 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-3GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859497
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51983 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM_E104Q-4GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859498
Proper citation: RRID:Addgene_51986 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_CaM-2GS
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GenBank accession number: JN859494
The minor discrepancies between the QC and depositor sequence have no functional consequence.
Proper citation: RRID:Addgene_51985 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: let-7 sequence for nucleotides 2-15 is CAACCTACTACCTCA.
Proper citation: RRID:Addgene_52039 Copy
Species: Synthetic
Genetic Insert: Nano-lantern_ATP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
References:
Comments: GenBank accession number: JN859502. Although they differ by only three N-terminal amino acidsin the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.
Proper citation: RRID:Addgene_51991 Copy
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