Plasmids   Select Another Resource Report Type

http://www.addgene.org/19549

Species: Homo sapiens
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family Disclaimer: For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.

Proper citation: RRID:Addgene_19549 Copy   

•   Source: Addgene

http://www.addgene.org/1970

Species: Mus musculus
Genetic Insert: mPh1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: Untagged full-length mouse PH1 cDNA for protein expression (kingston #1387)

Proper citation: RRID:Addgene_1970 Copy   

•   Source: Addgene

http://www.addgene.org/1971

Species: Mus musculus
Genetic Insert: Nore1
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11857081

Proper citation: RRID:Addgene_1971 Copy   

•   Source: Addgene

http://www.addgene.org/19751

Species: Mus musculus
Genetic Insert: oligo targeting p53
Vector Backbone Description: Backbone Marker:MSCV2.2 with WPRE cloned into ClaI site is the base vector; Backbone Size:7519; Vector Backbone:MSCV P2Gm; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: See FLIP vector protocols (PDF from this page) for more information. There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.

Proper citation: RRID:Addgene_19751 Copy   

•   Source: Addgene

http://www.addgene.org/19750

Genetic Insert: oligo targeting Firefly luciferase
Vector Backbone Description: Backbone Marker:MSCV2.2 with WPRE cloned into ClaI site is the base vector; Backbone Size:7518; Vector Backbone:MSCV P2Gm; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18779577
Comments: MSCV-based entry vector for cloning and validating miR30-based RNAi constructs. The vector expresses puromycin-resistance and GFP, separated by the FMDV 2A peptide. This is considered the "empty" vector and can be used for cloning. It contains a hairpin targeting Firefly luciferase. See FLIP vector protocols (PDF from this page) for more information. There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.

Proper citation: RRID:Addgene_19750 Copy   

•   Source: Addgene

http://www.addgene.org/1975

Species: Mus musculus
Genetic Insert: Nore1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11857081

Proper citation: RRID:Addgene_1975 Copy   

•   Source: Addgene

http://www.addgene.org/19754

Species: Homo sapiens
Genetic Insert: STIM1
Vector Backbone Description: Backbone Marker:See author's map; Backbone Size:6000; Vector Backbone:MO91; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16921383
Comments: See Author's Map for empty backbone (MO91) information.

Proper citation: RRID:Addgene_19754 Copy   

•   Source: Addgene

http://www.addgene.org/1967

Species: Mus musculus
Genetic Insert: Bmi1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: mouse Bmi1 cDNA with N-terminal Flag fusion. (kingston #1381)

Proper citation: RRID:Addgene_1967 Copy   

•   Source: Addgene

http://www.addgene.org/1968

Species: Mus musculus
Genetic Insert: Mel18
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: mouse Mel-18 cDNA with N-terminal Flag fusion. (kingston #1385)

Proper citation: RRID:Addgene_1968 Copy   

•   Source: Addgene

http://www.addgene.org/1969

Species: Mus musculus
Genetic Insert: Mel18
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14967148
Comments: mouse Mel-18 cDNA with C-terminal Flag fusion. (kingston #1386)

Proper citation: RRID:Addgene_1969 Copy   

•   Source: Addgene

http://www.addgene.org/19695

Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:LTRi; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17662949

Proper citation: RRID:Addgene_19695 Copy   

•   Source: Addgene

http://www.addgene.org/19730

Species: Homo sapiens
Genetic Insert: MKK7B2Jnk1a1(APF)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12052897

Proper citation: RRID:Addgene_19730 Copy   

•   Source: Addgene

http://www.addgene.org/19699

Species: Mus musculus
Genetic Insert: Merlin I
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pXJ40-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16247032

Proper citation: RRID:Addgene_19699 Copy   

•   Source: Addgene

http://www.addgene.org/19732

Vector Backbone Description: Backbone Size:9205; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: There are minor discrepancies between Addgene's quality control sequence and the reference sequence provided by the depositing lab. These discrepancies should not affect plasmid function.

Proper citation: RRID:Addgene_19732 Copy   

•   Source: Addgene

http://www.addgene.org/19735

Species: Bovine
Genetic Insert: alpha-FlincG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression, protein expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18165313

Proper citation: RRID:Addgene_19735 Copy   

•   Source: Addgene

http://www.addgene.org/19734

Species: Bovine
Genetic Insert: delta-FlincG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18165313

Proper citation: RRID:Addgene_19734 Copy   

•   Source: Addgene

http://www.addgene.org/19737

Genetic Insert: Cygnet 2.1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11226257

Proper citation: RRID:Addgene_19737 Copy   

•   Source: Addgene

http://www.addgene.org/19736

Species: Bovine
Genetic Insert: beta-FlincG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18165313

Proper citation: RRID:Addgene_19736 Copy   

•   Source: Addgene

http://www.addgene.org/19739

Species: Mus musculus
Genetic Insert: IkB kinase alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5044; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9520401

Proper citation: RRID:Addgene_19739 Copy   

•   Source: Addgene

http://www.addgene.org/19738

Species: Mus musculus
Genetic Insert: IkB kinase alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5044; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9520401
Comments: Splice variant should function the same as pCR-HA-IKKalpha

Proper citation: RRID:Addgene_19738 Copy   

•   Source: Addgene