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Species: Other
Genetic Insert: scaffold Sybody of the convex library
Vector Backbone Description: Vector Backbone:pBXPHM3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110104 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110107 Copy
Species: Homo sapiens
Genetic Insert: fibroblast growth factor receptor 2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6300; Vector Backbone:p3xFlag-CMV-13; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110108 Copy
Species: Clostridium difficile 70-100-2010
Genetic Insert: Pyrroline-5-carboxylate Reductase
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5292; Vector Backbone:pET-28 a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_110295 Copy
Species: Mus musculus
Genetic Insert: mcry1 K31R
Vector Backbone Description: Backbone Marker:genscript; Vector Backbone:pCDNA 3.1 HA tag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110297 Copy
Species: A. victoria
Genetic Insert: eGFPemerald
Vector Backbone Description: Backbone Marker:Unknown; Backbone Size:2700; Vector Backbone:pUC18; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: GFPemerald purchased from Packard.
Proper citation: RRID:Addgene_110210 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110215 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110216 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110214 Copy
Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Opher Gileadi, Addgene plasmid #26107; Vector Backbone:pNIC-ZB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110219 Copy
Species: Mus musculus
Genetic Insert: SRY (sex determining region Y)-box 2
Vector Backbone Description: Backbone Size:9501; Vector Backbone:pCWtre-hpaI-pacI-rtTAiBlast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110280 Copy
Species: A. victoria (jellyfish)
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pKS-ins:MCS2; cryaa:RFP; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: GCaMP6s fragment obtained from Addgene Plasmid Number: 40753 (pGP-CMV-GCaMP6s).
Proper citation: RRID:Addgene_110285 Copy
Species: Synthetic
Genetic Insert: Brainbow1.0L
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pKS-ins:MCS; cryaa:RFP; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
References:
Comments: Brainbow1.0L fragment obtained from Addgene Plasmid Number: 18725 (Thy1-Brainbow-1.0 L).
Proper citation: RRID:Addgene_110283 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6582; Vector Backbone:pKS-insulin_cryaa:RFP; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110286 Copy
Species: E. coli
Genetic Insert: yfaL
Vector Backbone Description: Backbone Size:3986; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern
Proper citation: RRID:Addgene_110287 Copy
Species: Homo sapiens
Genetic Insert: DENND1A
Vector Backbone Description: Backbone Marker:Nicola Burgess-Brown (Addgene plasmid # 39191); Vector Backbone:pFB-CT10HF-LIC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Use SF9 cells for expression. 6EKK: https://www.thesgc.org/structures/6EKK.
Proper citation: RRID:Addgene_110270 Copy
Species: Homo sapiens
Genetic Insert: Elongin BC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.
Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.
Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...
2C9W: https://www.thesgc.org/structures/2C9W.
Proper citation: RRID:Addgene_110274 Copy
Species: Homo sapiens
Genetic Insert: MTHFR
Vector Backbone Description: Backbone Marker:Nicola Burgess-Brown (Addgene plasmid # 39191); Vector Backbone:pFB-CT10HF-LIC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Use SF9 cells for expression. PDB: 6FCX
Proper citation: RRID:Addgene_110271 Copy
Species: Homo sapiens
Genetic Insert: ULK3
Vector Backbone Description: Backbone Marker:SGC Oxford; Vector Backbone:pFB-6HZB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Use SF9 cells for expression. PDB: 6FDY
Proper citation: RRID:Addgene_110272 Copy
Species: Mus musculus
Genetic Insert: chemokine (C-X-C motif) ligand 5
Vector Backbone Description: Backbone Size:11000; Vector Backbone:GFP.Cre empty vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_110278 Copy
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