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Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette
Proper citation: RRID:Addgene_22423 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3
Proper citation: RRID:Addgene_22424 Copy
Species: Drosophila melanogaster
Genetic Insert: Argonaut-1, variant A
Vector Backbone Description: Backbone Marker:Elisa Izaurralde; Backbone Size:5455; Vector Backbone:pac5.1B-lambdaN; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22420 Copy
Species: Homo sapiens
Genetic Insert: F6
Vector Backbone Description: Backbone Size:5854; Vector Backbone:pGL2 enhancer; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22438 Copy
Species: Homo sapiens
Genetic Insert: F6 Sp-1 mut
Vector Backbone Description: Backbone Size:5854; Vector Backbone:pGL2 enhancer; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Sp1 mutation (−103) oligo is - GGATAGGGACTGGGCGAGG
Proper citation: RRID:Addgene_22439 Copy
Species: Homo sapiens
Genetic Insert: F4
Vector Backbone Description: Backbone Size:5854; Vector Backbone:pGL2 enhancer; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22434 Copy
Species: Homo sapiens
Genetic Insert: F4 GATA mut
Vector Backbone Description: Backbone Size:5854; Vector Backbone:pGL2 enhancer; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: GATA mutation oigo -
GCTCCCACTTTAGAGCCTCAGT
Proper citation: RRID:Addgene_22435 Copy
Species: Homo sapiens
Genetic Insert: F5
Vector Backbone Description: Backbone Size:5854; Vector Backbone:pGL2 enhancer; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22436 Copy
Species: Homo sapiens
Genetic Insert: F3 GATA Sp-1 mut
Vector Backbone Description: Backbone Size:5854; Vector Backbone:pGL2 enhancer; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: The forward primer used for the Sp1 mutations (-103) was GGATAGGGACTGGGCGAGG.
GATA mutation oigo -
GCTCCCACTTTAGAGCCTCAGT.
Proper citation: RRID:Addgene_22431 Copy
Species: Homo sapiens
Genetic Insert: F3 Sp-1 mut
Vector Backbone Description: Backbone Size:5854; Vector Backbone:pGL2 enhancer; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Forward primer used to create Sp-1 mutation: GGATAGGGACTGGGCGAGG
Proper citation: RRID:Addgene_22432 Copy
Species: Homo sapiens
Genetic Insert: Human genomic PLP
Vector Backbone Description: Backbone Marker:Fermentas; Backbone Size:2862; Vector Backbone:pTZ19R; Vector Types:phagemid; Bacterial Resistance:Ampicillin
References:
Comments: Genomic DNA from an EMBL3 human genomic library. Contains 3' end of Exon1, Intron 1, Exon 2 and Intron 2
Proper citation: RRID:Addgene_22647 Copy
Species: Rattus norvegicus
Genetic Insert: Myelin Transcription Factor 1L
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2743; Vector Backbone:pGEM-3Z; Vector Types:Cloning and RNA Synthesis; Bacterial Resistance:Ampicillin
References:
Comments: Used to make riboprobes by cutting with SmaI and transcribing with SP6 polymerase to give a 980 bp riboprobe for RNA analysis on Northern blots
This Rat Myt1 DNA cloned by Hudson lab.
Proper citation: RRID:Addgene_22649 Copy
Species: Homo sapiens
Genetic Insert: BAP1
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22539 Copy
Species: Mus musculus
Genetic Insert: Proteolipid Protein
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2867; Vector Backbone:pGEM3; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
References:
Comments: Contains entire coding region of the mouse PLP cDNA including part of the 5' UTR. Used to make riboprobes by transcribing with SP6 polymerase to give a 980 bp antisense riboprobe or transcribing with T7 polymerase to give a 980bp sense riboprobe for RNA analysis on Northern blots.
This PLP DNA cloned by Hudson lab.
Proper citation: RRID:Addgene_22651 Copy
Species: Mus musculus
Genetic Insert: Ror2 delta Ig
Vector Backbone Description: Backbone Size:9226; Vector Backbone:pEF1a-myc/his A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22629 Copy
Species: Homo sapiens
Genetic Insert: human genomic PLP promoter region
Vector Backbone Description: Backbone Marker:R. McKinnon; Backbone Size:4728; Vector Backbone:p18CAT; Vector Types:CAT reporter plasmid; Bacterial Resistance:Ampicillin
References:
Comments: Insert is 4200bp (approx) of the Human PLP 5' upstream, promoter region and Exon1 (-4200 to +85) in the correct orientation. Insert is a BamHI/HindIII from pJB010
This PLP DNA cloned by Hudson lab.
Proper citation: RRID:Addgene_22624 Copy
Species: Homo sapiens
Genetic Insert: human genomic PLP promoter region
Vector Backbone Description: Backbone Marker:R. McKinnon; Backbone Size:4728; Vector Backbone:p18CAT; Vector Types:CAT reporter plasmid; Bacterial Resistance:Ampicillin
References:
Comments: Insert is 30bp ) of the Human PLP 5' upstream, promoter region and Exon1 (-18 to +12) in the correct orientation.
This PLP DNA cloned by Hudson lab.
Proper citation: RRID:Addgene_22625 Copy
Species: Homo sapiens
Genetic Insert: human genomic PLP promoter region
Vector Backbone Description: Backbone Marker:R. McKinnon; Backbone Size:4728; Vector Backbone:p18CAT; Vector Types:CAT reporter plasmid; Bacterial Resistance:Ampicillin
References:
Comments: Insert is 1200bp (approx) of the Human PLP 5' upstream, promoter region and Exon1 (-1088 to +85) in the correct orientation. Site 3 (bp -117 to -116) were mutated via Site-Directed Mutagenesis from GG to cc.
This PLP DNA cloned by Hudson lab.
Proper citation: RRID:Addgene_22626 Copy
Species: Homo sapiens
Genetic Insert: USP50
Vector Backbone Description: Backbone Marker:Harper_Lab; Backbone Size:6521; Vector Backbone:pDEST_LTR_N_FLAG_HA_IRES_puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22588 Copy
Species: Homo sapiens
Genetic Insert: human genomic PLP promoter region
Vector Backbone Description: Backbone Marker:R. McKinnon; Backbone Size:4728; Vector Backbone:p18CAT; Vector Types:CAT reporter plasmid; Bacterial Resistance:Ampicillin
References:
Comments: Insert is 1200bp (approx) of the Human PLP 5' upstream, promoter region and Exon1 (-1088 to +85) in the correct orientation
This PLP DNA cloned by Hudson lab.
Proper citation: RRID:Addgene_22623 Copy
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