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Species: Synthetic
Genetic Insert: hTfR-GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37098770
Comments: Please visit https://doi.org/10.1101/2023.03.03.531050 for bioRxiv preprint. In the original study XL1 Blue E. coli are used for amplifying plasmids.
Proper citation: RRID:Addgene_199714 Copy
Species: Mus musculus
Genetic Insert: Ascl1
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pMX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36206732
Proper citation: RRID:Addgene_198227 Copy
Genetic Insert: opCas12a-T8N
Vector Backbone Description: Backbone Size:6086; Vector Backbone:Twinstrep-SUMO-huLwCas13a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37095348
Comments: For opCas12a-T8N bacterial expression, host stain Rosetta 2(DE3)pLysS was used.
Proper citation: RRID:Addgene_199605 Copy
Species: Homo sapiens
Genetic Insert: EWSR1
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_001163287,ENST00000333395 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_142470 Copy
Species: Homo sapiens
Genetic Insert: shCSE1L v1
Vector Backbone Description: Backbone Marker:Addgene #21915; Vector Backbone:tet pLKO.1 puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192345 Copy
Species: Homo sapiens
Genetic Insert: shNUP37 v2
Vector Backbone Description: Backbone Marker:Addgene #21915; Vector Backbone:tet pLKO.1 puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192344 Copy
Species: Homo sapiens
Genetic Insert: shCLTC v2
Vector Backbone Description: Backbone Marker:Addgene #21915; Vector Backbone:tet pLKO.1 puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192348 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: shCse1l v1
Vector Backbone Description: Backbone Marker:Addgene #21915; Vector Backbone:tet pLKO.1 puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192342 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9.D10A nickase
Vector Backbone Description: Backbone Size:4965; Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398349
Comments: The insert SpCas9 was modified via the introduction of the RuvC nuclease domain-disabling mutation D10A and placed in a pCAG.rBGpA expression plasmid backbone.
Proper citation: RRID:Addgene_199256 Copy
Species: Streptococcus pyogenes
Genetic Insert: High specificity S. pyogenes Cas9-D10A-KARA nickase
Vector Backbone Description: Backbone Size:4965; Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398349
Proper citation: RRID:Addgene_199254 Copy
Species: Homo sapiens
Genetic Insert: TLR4
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_197640 Copy
Species: Caenorhabditis elegans
Genetic Insert: 15xUAS::lmp1-venus::let-858 3'UTR
Vector Backbone Description: Backbone Marker:Fire Lab C. elegans Vector Kit; Vector Backbone:pPD117.01; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096407
Proper citation: RRID:Addgene_198824 Copy
Species: Caenorhabditis elegans
Genetic Insert: PF35D11.1-NLS-cGAL(DBD)-gp41-1-N-intein
Vector Backbone Description: Backbone Size:3704; Vector Backbone:pAH35; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096407
Proper citation: RRID:Addgene_198789 Copy
Species: Homo sapiens
Genetic Insert: KPNA1
Vector Backbone Description: Backbone Marker:Addgene #25736; Vector Backbone:pSLIK zeo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192329 Copy
Species: Homo sapiens
Genetic Insert: S. aureus gRNA spacer
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928106
Comments: This gRNA expression construct can be combined with target site-containing donor plasmids AD59_pEP.DonorCLYBL.TS (Addgene plasmid #199227) or AZ64_pE.DonorCLYBL.TS (Addgene plasmid #199228) to insert foreign DNA at the human CLYBL safe harbor locus using SaCas9 nucleases (HMEJ-based gene targeting) or SaCas9.D10A nickases (ITPN-based gene targeting), such as that encoded by Addgene plasmid #199251.
Proper citation: RRID:Addgene_199237 Copy
Species: Mus musculus
Genetic Insert: BAF53b
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is a validated antigen plasmid of Anti-BAF53b [N332B/15R].
Proper citation: RRID:Addgene_197297 Copy
Species: Caenorhabditis elegans
Genetic Insert: Ptrx-1-GAL4-SK(DBD)-VP64
Vector Backbone Description: Backbone Size:3611; Vector Backbone:pHW393; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38096407
Proper citation: RRID:Addgene_198781 Copy
Species: Homo sapiens
Genetic Insert: BirA*-3xFLAG-CSE1L
Vector Backbone Description: Backbone Marker:Addgene #25736; Vector Backbone:pSLIK zeo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192334 Copy
Species: Homo sapiens
Genetic Insert: NUP37
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192299 Copy
Species: Homo sapiens
Genetic Insert: mCh-V5-NES-EGFP-ERBB2 NLS
Vector Backbone Description: Backbone Marker:Addgene #25736; Vector Backbone:pSLIK zeo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192338 Copy
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