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Species: E. coli
Genetic Insert: birA
Vector Backbone Description: Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: Encodes untagged biotin ligase gene (birA) from E. coli fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used to purify biotin ligase.
Proper citation: RRID:Addgene_38258 Copy
Species: E. coli
Genetic Insert: PheS Gly294
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:13167; Vector Backbone:pGIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21307310
Proper citation: RRID:Addgene_44011 Copy
Species: E. coli
Genetic Insert: BirA::GFP
Vector Backbone Description: Backbone Size:2884; Vector Backbone:pWormgate2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22219512
Proper citation: RRID:Addgene_45100 Copy
Species: e. coli
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4102; Vector Backbone:pBAD/His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45397 Copy
Species: e. coli
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45392 Copy
Species: e. coli
Genetic Insert: tet repressor
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45391 Copy
Species: e. coli
Genetic Insert: deGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45394 Copy
Species: E. coli
Genetic Insert: rpoN
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341441
Proper citation: RRID:Addgene_41748 Copy
Species: E. coli
Genetic Insert: beta-Galactosidase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_45967 Copy
Species: E. coli
Genetic Insert: beta-Galactosidase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_45964 Copy
Species: E. coli
Genetic Insert: ECK120033127 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46011 Copy
Species: E. coli
Genetic Insert: ECK120010800 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46010 Copy
Species: E. coli
Genetic Insert: BBa_B0010 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46003 Copy
Species: E. coli
Genetic Insert: ECK125108723 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46008 Copy
Species: E. coli
Genetic Insert: ECK120010783 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46009 Copy
Species: E. coli
Genetic Insert: ECK120015170 Terminator
Vector Backbone Description: Backbone Size:5047; Vector Backbone:pGR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23727987
Proper citation: RRID:Addgene_46006 Copy
Species: E. coli
Genetic Insert: Streptavidin-Dead Aspartate Loop
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056174
Proper citation: RRID:Addgene_46368 Copy
Species: E. coli
Genetic Insert: GFP-LacI**
Vector Backbone Description: Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:5650; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21724830
Comments: The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser).
The plasmid pAT222 was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase):
5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and
5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’.
Alternate plasmid name: pAT123LacI2G(4mutations)
Proper citation: RRID:Addgene_40942 Copy
Species: E. coli
Genetic Insert: LexAp65
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR P4r-P3r; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_41438 Copy
Species: E. coli
Genetic Insert: EcoRI endonuclease & EcoRI methylase
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pSCC3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2745418
Comments: pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_40190 Copy
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