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Species: Homo sapiens
Genetic Insert: SPRTN
Vector Backbone Description: Backbone Marker:Opher Gileadi, Addgene plasmid #26107; Vector Backbone:pNIC-ZB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27871366
Comments: According to the large datasets deposited in various databses like: dbSNP, 1000 Genomes, the ExAC and the gnomAD browser, the variant c.887C>T, p.Pro296Leu is found roughly in 50% of general world population. Moreover, according to the large-scale sequencing studies involving more than 135 000 healthy individuals deposited in The Genome Aggregation Database (gnomAD), it is more prevalent than the previously assigned reference allele in the European population, with a minor allele frequency of 0.69: http://gnomad.broadinstitute.org/variant/1-231488524-C-T
Taken this into account, this variant (P296L) is s actually the accurate reference (wild-type) allele, and thus this variant can not be the cause of any Mendelian disease. This is the reason why all our vectors bear this variant.
Proper citation: RRID:Addgene_110219 Copy
Species: Mus musculus
Genetic Insert: SRY (sex determining region Y)-box 2
Vector Backbone Description: Backbone Size:9501; Vector Backbone:pCWtre-hpaI-pacI-rtTAiBlast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30332632
Proper citation: RRID:Addgene_110280 Copy
Species: A. victoria (jellyfish)
Genetic Insert: GCaMP6s
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pKS-ins:MCS2; cryaa:RFP; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939870
Comments: GCaMP6s fragment obtained from Addgene Plasmid Number: 40753 (pGP-CMV-GCaMP6s).
Proper citation: RRID:Addgene_110285 Copy
Species: Synthetic
Genetic Insert: Brainbow1.0L
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pKS-ins:MCS; cryaa:RFP; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939870
Comments: Brainbow1.0L fragment obtained from Addgene Plasmid Number: 18725 (Thy1-Brainbow-1.0 L).
Proper citation: RRID:Addgene_110283 Copy
Vector Backbone Description: Backbone Size:6582; Vector Backbone:pKS-insulin_cryaa:RFP; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28939870
Proper citation: RRID:Addgene_110286 Copy
Species: E. coli
Genetic Insert: yfaL
Vector Backbone Description: Backbone Size:3986; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22344647
Comments: Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern
Proper citation: RRID:Addgene_110287 Copy
Species: Homo sapiens
Genetic Insert: DENND1A
Vector Backbone Description: Backbone Marker:Nicola Burgess-Brown (Addgene plasmid # 39191); Vector Backbone:pFB-CT10HF-LIC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Use SF9 cells for expression. 6EKK: https://www.thesgc.org/structures/6EKK.
Proper citation: RRID:Addgene_110270 Copy
Species: Homo sapiens
Genetic Insert: Elongin BC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.
Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.
Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...
2C9W: https://www.thesgc.org/structures/2C9W.
Proper citation: RRID:Addgene_110274 Copy
Species: Homo sapiens
Genetic Insert: MTHFR
Vector Backbone Description: Backbone Marker:Nicola Burgess-Brown (Addgene plasmid # 39191); Vector Backbone:pFB-CT10HF-LIC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Use SF9 cells for expression. PDB: 6FCX
Proper citation: RRID:Addgene_110271 Copy
Species: Homo sapiens
Genetic Insert: ULK3
Vector Backbone Description: Backbone Marker:SGC Oxford; Vector Backbone:pFB-6HZB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Use SF9 cells for expression. PDB: 6FDY
Proper citation: RRID:Addgene_110272 Copy
Species: Mus musculus
Genetic Insert: chemokine (C-X-C motif) ligand 5
Vector Backbone Description: Backbone Size:11000; Vector Backbone:GFP.Cre empty vector; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30332632
Proper citation: RRID:Addgene_110278 Copy
Species: Synthetic
Genetic Insert: gHH_44
Vector Backbone Description: Backbone Size:5919; Vector Backbone:pCDB364; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30152054
Proper citation: RRID:Addgene_110276 Copy
Species: Homo sapiens
Genetic Insert: GLS Glutaminase
Vector Backbone Description: Backbone Marker:#85208; Backbone Size:7032; Vector Backbone:pLKO.1-TRC.mKO2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29115931
Comments: See Addgene's pLKO family protocol http://www.addgene.org/plko on how to use the pLKO family vectors.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_110319 Copy
Species: Homo sapiens
Genetic Insert: DPYSL4
Vector Backbone Description: Backbone Marker:SGC Oxford; Vector Backbone:pFB-6HZB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Use SF9 cells for expression. 5NKS: https://www.thesgc.org/structures/5NKS.
Proper citation: RRID:Addgene_110263 Copy
Species: Homo sapiens
Genetic Insert: WNK3
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26108); Vector Backbone:pFB-LIC-Bse; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Use SF9 cells for expression. 5O2C: https://www.thesgc.org/structures/5O2C.
Proper citation: RRID:Addgene_110261 Copy
Species: Homo sapiens
Genetic Insert: p53-binding protein 1
Vector Backbone Description: Backbone Marker:Feng Zhang, Addgene plasmid #48139; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253795
Proper citation: RRID:Addgene_110300 Copy
Species: Homo sapiens
Genetic Insert: LIMK2
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26108); Vector Backbone:pFB-LIC-Bse; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Use SF9 cells for expression. 5NXD: https://www.thesgc.org/structures/5NXD.
Proper citation: RRID:Addgene_110264 Copy
Species: Homo sapiens
Genetic Insert: ACVR1
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26108); Vector Backbone:pFB-LIC-Bse; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Use SF9 cells for expression. 6EIX: https://www.thesgc.org/structures/6EIX.
Proper citation: RRID:Addgene_110269 Copy
Species: Homo sapiens
Genetic Insert: WNK2
Vector Backbone Description: Backbone Marker:Opher Gileadi (Addgene plasmid # 26103); Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Use BL21(DE3)-R3-pRARE2 for expression. 6ELM: https://www.thesgc.org/structures/6ELM.
Proper citation: RRID:Addgene_110250 Copy
Species: Caenorhabditis elegans
Genetic Insert: End-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:xenopus, mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10760276
Proper citation: RRID:Addgene_11030 Copy
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