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Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11164; Vector Backbone:pLEXiPuro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48956 Copy
Vector Backbone Description: Backbone Marker:Rajewsky Lab, PMID 18327259 ; Vector Backbone:pSiP1; Vector Types:Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Comments: The presence of firefly luciferase in the psiCHECK-2-derived vectors allows normalization of the Renilla luciferase expression that monitors the RNAi effect.
Proper citation: RRID:Addgene_48955 Copy
Vector Backbone Description: Backbone Marker:Imre Berger; Backbone Size:7720; Vector Backbone:pFBDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26043233
Comments: Constructed by Christopher Weinert, Grütter lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49051 Copy
Genetic Insert: TALE-TF(VP64)-BB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Adeno-associated Virus (); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22828628
Comments: This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
Proper citation: RRID:Addgene_42581 Copy
Genetic Insert: TALEN-FokI(WT)-BB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Adeno-associated Virus (); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22828628
Comments: This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
Proper citation: RRID:Addgene_42588 Copy
Vector Backbone Description: Backbone Marker:Elledge; Backbone Size:11791; Vector Backbone:pHAGE-TRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21307310
Comments: pHAGE-TRE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)
Proper citation: RRID:Addgene_44012 Copy
Vector Backbone Description: Backbone Marker:Addgene Plasmid 19778; Backbone Size:8600; Vector Backbone:FU-tet-o-hOct4; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22174877
Comments: To make a drug-inducible lentiviral vector that is compatible with Gateway recombination (Invitrogen), the depositing laboratory removed the OCT4 open reading frame from FU-tetO-hOCT4 (Addgene plasmid 19778) and replaced it with a Gateway cassette cloned from pEF-DEST51 (Invitrogen) to generate FU-tetO-Gateway. ORFs that lack stop codons can be cloned into this plasmid to express proteins with a C-terminal V5 epitope tag.
Please note that Addgene's sequencing results identified a single nucleotide insert between bp# 2913/2914 and single nucleotide mismatches at bp# 3303 and 4652 when compared to the full plasmid sequence provided by the depositing laboratory. These differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_43914 Copy
Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1, NeoR, [4-3] Gateway
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4070; Vector Backbone:pDESTR4-R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44473 Copy
Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1, NeoR, peel-1, [4-3] Gateway
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4070; Vector Backbone:pDESTR4-R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44477 Copy
Species: Drosophila melanogaster
Genetic Insert: Minimal Mos1, peel-1, [4-3] Gateway
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4070; Vector Backbone:pDESTR4-R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44479 Copy
Species: C. Briggsae
Genetic Insert: Minimal Mos1, unc-119, [4-3] Gateway
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4070; Vector Backbone:pDESTR4-R3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Proper citation: RRID:Addgene_44472 Copy
Species: Synthetic
Genetic Insert: sgRNA-scaffold only
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:2125; Vector Backbone:pMD19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35969224
Proper citation: RRID:Addgene_190794 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28060929
Proper citation: RRID:Addgene_190226 Copy
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_194066 Copy
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_194073 Copy
Species: HIV1
Genetic Insert: p55 GAG
Vector Backbone Description: Backbone Size:2956; Vector Backbone:pMET7; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27122307
Proper citation: RRID:Addgene_194634 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71500 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71501 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71505 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: We replaced the SV40 promotor of the pGL3-promotor plasmid by DSCP and inserted a Gateway-cassette into the multiple-cloning-site (MCS; using KpnI and BglII), i.e. upstream of the DSCP, to allow Gateway cloning of candidate sequences
Proper citation: RRID:Addgene_71506 Copy
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