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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pDEST myc MOV10 D645N
 
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RRID:Addgene_51720 MOV10 D645N Homo sapiens Ampicillin PMID:24726324 Backbone Marker:Invitrogen; Backbone Size:5882; Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D645N 2026-08-01 01:14:27 0
pcDNA3.1-aDTX-lumitoxin
 
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RRID:Addgene_51686 aDTX-lumitoxin Synthetic Ampicillin PMID:24407101 Backbone Size:5341; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:26 0
SpyLigase
 
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1+ mentions
RRID:Addgene_51722 SpyLigase Synthetic Ampicillin PMID:24639550 SnoopLigase is the more recent peptide-peptide ligation technology from the Howarth lab and is now preferred over SpyLigase in terms of more general reaction conditions and higher coupling yield on a range of proteins. pET28a-AviTag-SnoopLigase https://www.addgene.org/105626/ pET28a-HaloTag7-SnoopLigase https://www.addgene.org/105627/ SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Jacob O. Fierer, Gianluca Veggiani and Mark Howarth Transform plasmid into BL21 DE3 RIPL for expression. Backbone Marker:Life Technologies; Backbone Size:6400; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 2
p18S.1 G693A
 
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RRID:Addgene_51728 18S Mus musculus Ampicillin PMID:22718970 Please note that the depositor sequence is theoretical. Addgene verified that the nucleotides A5152 (nucleotide 963 in 18 S rRNA) and G5162 are expected in 18S, based on the publication and GenBank ID: JQ247698.1. Please see Addgene's result using the m18Sinternal-rev primer in the "Sequences" link above. Backbone Marker:promega; Backbone Size:2000; Vector Backbone:pCMV; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
DRH296: FCK-Optopatch2
 
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1+ mentions
RRID:Addgene_51694 Optopatch2 Synthetic Ampicillin PMID:24952910 Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 8
DRH335: FCK-Optopatch1
 
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1+ mentions
RRID:Addgene_51695 Optopatch1 Synthetic Ampicillin PMID:24952910 Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 2
pCDNA3-ySet2
 
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RRID:Addgene_51698 yeast Set2 Saccharomyces cerevisiae Ampicillin PMID:20133523 Backbone Marker:commercial; Backbone Size:5400; Vector Backbone:pcDNA3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
R5 EMCV
 
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1+ mentions
RRID:Addgene_51733 EMCV IRES Ampicillin PMID:22718970 Backbone Marker:Promega; Backbone Size:4600; Vector Backbone:pGl4.13; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 2
R5 MCS
 
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RRID:Addgene_51732 MCS Ampicillin PMID:22718970 Backbone Marker:Promega; Backbone Size:4600; Vector Backbone:pGl4.13; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
p413TEF-SirT1
 
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1+ mentions
RRID:Addgene_51746 SirT1 Homo sapiens Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 2
p413TEF-Sir2
 
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RRID:Addgene_51742 Sir2 Saccharomyces cerevisiae Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
ESAM-bio-His
 
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RRID:Addgene_51751 ESAM Homo sapiens Ampicillin PMID:25713122 Article ref 20 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
EPHB1-bio-His
 
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1+ mentions
RRID:Addgene_51750 EPHB1 Homo sapiens Ampicillin PMID:25713122 Article ref 19 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 1
ICAM5-bio-His
 
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RRID:Addgene_51759 ICAM5 Homo sapiens Ampicillin PMID:25713122 Article ref 28 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
ICAM2-bio-His
 
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RRID:Addgene_51758 ICAM2 Homo sapiens Ampicillin PMID:25713122 Article ref 27 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
FCGR2a-bio-His
 
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RRID:Addgene_51754 FCGR2a Homo sapiens Ampicillin PMID:25713122 Article ref 22 Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 0
lentiCRISPR - EGFP sgRNA 3
 
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1+ mentions
RRID:Addgene_51762 Cas9 Synthetic Ampicillin PMID:24336571 gRNA target sequence GGCCACAAGTTCAGCGTGTC These six EGFP-targeting lentiCRISPRs have EGFP-targeting sgRNAs cloned into the lentiCRISPR backbone plasmid (Addgene plasmid 49535) and are used in Fig 1B of Shalem*, Sanjana* et al (2014). If you want to clone your own targeting sequences, please use the lentiCRISPR backbone plasmid (Addgene plasmid 49535), as the sgRNA Type IIs cloning site is not present in these plasmids. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information. Backbone Marker:Zhang lab; Vector Backbone:lentiCRISPR (pXPR_001), Addgene plasmid #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 2
pCDNA3 T7 Jip1(delta JBD)
 
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RRID:Addgene_51767 Jnk interacting protein 1 Mus musculus Ampicillin PMID:9235893 Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin deleted 126-263 2026-08-01 01:14:27 0
RP1B-PP1beta
 
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1+ mentions
RRID:Addgene_51769 pp1 beta Kanamycin Backbone Marker:Peti Lab (Addgene Plasmid# 26563); Vector Backbone:RP1B; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Contains PP1 beta residues 6-327 2026-08-01 01:14:27 1
lentiCRISPR - EGFP sgRNA 4
 
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1+ mentions
RRID:Addgene_51763 Cas9 Synthetic Ampicillin PMID:24336571 gRNA target sequence GGAGCGCACCATCTTCTTCA These six EGFP-targeting lentiCRISPRs have EGFP-targeting sgRNAs cloned into the lentiCRISPR backbone plasmid (Addgene plasmid 49535) and are used in Fig 1B of Shalem*, Sanjana* et al (2014). If you want to clone your own targeting sequences, please use the lentiCRISPR backbone plasmid (Addgene plasmid 49535), as the sgRNA Type IIs cloning site is not present in these plasmids. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check https://zlab.bio/ for the most up-to-date information. Backbone Marker:Zhang lab; Vector Backbone:lentiCRISPR (pXPR_001), Addgene plasmid #49535; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-01 01:14:27 4

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