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Species: Homo sapiens
Genetic Insert: OR51E2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22324 Copy
Species: Homo sapiens
Genetic Insert: OR5P3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22326 Copy
Species: Homo sapiens
Genetic Insert: OR2C1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22320 Copy
Species: Mus musculus
Genetic Insert: MOR33-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22338 Copy
Species: Mus musculus
Genetic Insert: MOR23-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22334 Copy
Species: Mus musculus
Genetic Insert: MOR31-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The sequence I sent is a 100% match to AY317748 (a variant of MOR31-1) and is the variant we used in the paper. L178H and Y10D are apparent in other variants.
Proper citation: RRID:Addgene_22337 Copy
Species: Mus musculus
Genetic Insert: MOR5-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22330 Copy
Species:
Genetic Insert: SV40 small + Large T antigen
Vector Backbone Description: Backbone Size:6925; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: The small and large T antigen inactivates the TP53, RB1 and PP2A genes.
Proper citation: RRID:Addgene_22298 Copy
Species: Mus musculus
Genetic Insert: MOR9-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22331 Copy
Species: Mus musculus
Genetic Insert: MOR15-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This clone was originally identified as Olfr611, but upon resequencing, it is actually Olfr612.
Proper citation: RRID:Addgene_22332 Copy
Species: Mus musculus
Genetic Insert: MOR18-1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22333 Copy
Species: Homo sapiens
Genetic Insert: Synaptojanin 1_145
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_22293 Copy
Species: Saccharomyces castellii
Genetic Insert: DCR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5753; Vector Backbone:pET101/D-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22309 Copy
Species: Mus musculus
Genetic Insert: Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:0; Vector Backbone:pET42a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_22388 Copy
Species:
Genetic Insert: GFP-rad9-GFP
Vector Backbone Description: Backbone Size:4456; Vector Backbone:pRS403; Vector Types:Yeast Expression, Integrating plasmid; Bacterial Resistance:Ampicillin
References:
Comments: GAL1 promoter, GFP silencing construct and CYC1 terminator fragment was cloned out of pYES2.1 and into pRS403.
The insert sequence can be found by clicking on the "View sequence" link. The rad9 intron loop is shown in upper case letters.
Proper citation: RRID:Addgene_22316 Copy
Species:
Genetic Insert: URA hairpin
Vector Backbone Description: Backbone Size:4456; Vector Backbone:pRS403; Vector Types:Yeast Expression, Integrating plasmid; Bacterial Resistance:Ampicillin
References:
Comments: 339bp of URA3 sequence from pRS406 was cloned into pYES2.1 GFP weak silencing construct in the sense orientation between PvuII and XhoI (replacing the GFP) and in antisense between EcoRI and XbaI sites. The expression cassette containing GAL1 promoter, URA3 silencing construct and CYC1 terminator was moved into pRS403.
The URA3 insert sequence can be found by clicking on the "View sequence" link. The rad9 intron loop is shown in upper case letters.
Please note that Addgene's sequencing results found a single nucleotide mismatch in the URA3 sequence at bp#3470 when compared to the full plasmid sequence provided by the depositing laboratory. This mismatch is not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_22317 Copy
Species: Homo sapiens
Genetic Insert: OR10J5
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_22318 Copy
Species: Saccharomyces castellii
Genetic Insert: AGO1
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS404-PTEF; Vector Types:Yeast Expression, Integrating plasmid; Bacterial Resistance:Ampicillin
References:
Comments: AgoI coding sequence was inserted between TEF promoter and CYC1 terminator.
Proper citation: RRID:Addgene_22313 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5857; Vector Backbone:pYES2.1 modified; Vector Types:Yeast Expression, Integrating plasmid; Bacterial Resistance:Ampicillin
References:
Comments: A MCS containing XhoI and EcoRI sites was cloned between the PvuII and XbaI sites of pYES2.1. The empty vector was created by replacing the hairpin of pIp-strongSC_GFP with XhoI and EagI sites
To make this plasmid integrating, the 2micron and f1 origins were replaced (using NheI and SpeI sites with sequence from S. castellii sc633:288301-289016 (amplified from genomic DNA with 5'-AAAAGCTAGCGATCCCTTATCAAATATGGTAC and 5'-AAAAACTAGTGTAGAATCCAGAGAATAGAATC).
Please note that Addgene's sequencing results differ from the full plasmid sequence from the depositing laboratory by a single nucleotide mismatch at bp# 3135 in the vector backbone. This mismatch is not known to affect plasmid function.
Proper citation: RRID:Addgene_22310 Copy
Species: Mus musculus
Genetic Insert: Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:0; Vector Backbone:pET42a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_22392 Copy
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