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Species: Synthetic
Genetic Insert: plamodium falciparum TRAP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23236185
Proper citation: RRID:Addgene_50048 Copy
Species: Synthetic
Genetic Insert: pfTRAP(vwa)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23236185
Proper citation: RRID:Addgene_50049 Copy
Species: Synthetic
Genetic Insert: G-CaMP3
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23364746
Comments: NOTE: There are some discrepancies between the Addgene quality control sequence and the depositor's full assembled sequence. These differences are not in functionally relevant parts of the plasmid.
Proper citation: RRID:Addgene_50022 Copy
Species: Synthetic
Genetic Insert: Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
Vector Backbone Description: Backbone Size:4086; Vector Backbone:pCS2+; Vector Types:Multi-purpose expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_50020 Copy
Species: Synthetic
Genetic Insert: p9-FLAG-FMO
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:CDF-Duet; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27487319
Proper citation: RRID:Addgene_86479 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:6181; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27914197
Proper citation: RRID:Addgene_86537 Copy
Species: Synthetic
Genetic Insert: p9-6His-tnaA
Vector Backbone Description: Vector Backbone:pWW307; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27487319
Proper citation: RRID:Addgene_86481 Copy
Species: Synthetic
Genetic Insert: Pveg-sLucopt
Vector Backbone Description: Backbone Marker:Robert Fagan and Neil Fairweather; Backbone Size:6378; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that the individual components of this construct (that is, Pveg and sLucopt) are described in Oliveira Paiva AM et al (2016) ACS Synth Biol (DOI: 10.1021/acssynbio.6b00104). During the cloning a mutation occurred that removes the SacI site in between the Pveg and sLucopt genes. The 5' (KpnI) and 3' (BamHI) restriction sites remain.
Proper citation: RRID:Addgene_86554 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kristin Bernhardt; Backbone Size:11630; Vector Backbone:pKB65; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28174584
Comments: See Hahn et al., 2017
Proper citation: RRID:Addgene_86556 Copy
Species: Synthetic
Genetic Insert: CANE-LV envelope
Vector Backbone Description: Backbone Size:4821; Vector Backbone:pCASGS/ES; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27974160
Proper citation: RRID:Addgene_86666 Copy
Species: Synthetic
Genetic Insert: MTST6
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2700; Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060124
Proper citation: RRID:Addgene_86603 Copy
Species: Synthetic
Genetic Insert: ER-GCaMP6-210
Vector Backbone Description: Backbone Size:9246; Vector Backbone:FCK(1.3)GW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28162809
Proper citation: RRID:Addgene_86919 Copy
Species: Synthetic
Genetic Insert: mKO2-hCDT1(30-120)
Vector Backbone Description: Backbone Marker:Verma lab (Salk Institute); Backbone Size:7800; Vector Backbone:pBOB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27888217
Proper citation: RRID:Addgene_86849 Copy
Species: Synthetic
Genetic Insert: NS3 1-177
Vector Backbone Description: Backbone Marker:Novagene; Backbone Size:5420; Vector Backbone:pETDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27940580
Proper citation: RRID:Addgene_86846 Copy
Species: Synthetic
Genetic Insert: Cas9-venus
Vector Backbone Description: Backbone Size:2770; Vector Backbone:Bluescript; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30912045
Proper citation: RRID:Addgene_86986 Copy
Species: Synthetic
Genetic Insert: PCNA3 fused to phosphite dehydrogenase and P450cam
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86971 Copy
Species: Synthetic
Genetic Insert: PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86969 Copy
Species: Synthetic
Genetic Insert: PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET-15b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733255
Proper citation: RRID:Addgene_86968 Copy
Species: Synthetic
Genetic Insert: mTagBFP-KDEL
Vector Backbone Description: Backbone Size:5689; Vector Backbone:pEFIRES-P; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27879284
Comments: This vector was generated by Simon Pfisterer (Elina Ikonen Lab).
The BFP-KDEL derived from addgene plasmid #49150 was transferred to pEFIRES-P vector to facilitate the selection of stable mammalian cell lines.
Benefits of using pEFIRES-P vector for stable overexpression in mammalian cells were described by Stephen Hobbs et al. (Biochem Biophys Res Commun. 1998 Nov 18;252(2):368-72.).
pEFIRES-P was a gift from Dr. Olli Ritvos (University of Helsinki).
Proper citation: RRID:Addgene_87163 Copy
Species: Synthetic
Genetic Insert: 1BPNLS-Cas9-1BPNLS-2A-EGFP
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27851729
Comments: The amino acid sequence of BPSV40NLS is "KRTADGSEFESPKKKRKV"
Proper citation: RRID:Addgene_87109 Copy
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