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Species: Medicago truncatula
Genetic Insert: MT4Noble_004794.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164754 Copy
Species: Medicago truncatula
Genetic Insert: Medtr3g067445.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164751 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSAR-MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The pSAR-MT inducible
expression plasmid was constructed as follows. Two
oligonucleotides (5'-GATCTCGAGCTCCCTGCA-3' and 5'-GGGAGCTCGA-3') were annealed, and the resulting fragment was cloned into the BamHI and PstI sites of the scaffold attachment region (SAR)-containing plasmid p8. The resulting plasmid, pJM7, consisted of two SAR sequences
flanking a short polylinker containing restriction sites for Xhol and PstI. The mutant metallothionein (MT) promoter was amplified by PCR and cloned into pCEP4, linearized
with BglII and NotI, yielding pCEP-MT. A SalI-BamHI
fragment containing an intron from the globin gene was then
inserted into the XhoI and BamHI sites of pCEP-MT, yielding pCEP-MTi. Finally, the MT promoter/intron/poly(A) region was removed from pCEP-MTi by digestion with Sall and cloned into the XhoI site of pJM7, yielding pSAR-MT.
Proper citation: RRID:Addgene_16485 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16483 Copy
Species: Medicago truncatula
Genetic Insert: Medtr3g467100.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Peter/Shulan, additional dsRED selection
Proper citation: RRID:Addgene_164758 Copy
Species: Medicago truncatula
Genetic Insert: Medtr1g080990.2
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Peter/Shulan, additional dsRED selection
Proper citation: RRID:Addgene_164755 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-3
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164860 Copy
Species: Medicago truncatula
Genetic Insert: Medtr3g094130.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164743 Copy
Species: Leptotrichia buccalis
Genetic Insert: AcrVIA7
Vector Backbone Description: Backbone Size:4010; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: The anti-CRISPR protein AcrVIA7 was codon-optimized for E. coli for optimal expression.
Proper citation: RRID:Addgene_164864 Copy
Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-3
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164867 Copy
Species: Medicago truncatula
Genetic Insert: Medtr3g095690.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164744 Copy
Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-2
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164866 Copy
Species: Medicago truncatula
Genetic Insert: Medtr8g442670.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164771 Copy
Species: Medicago truncatula
Genetic Insert: Medtr2g100600.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164772 Copy
Species:
Genetic Insert: GluN2A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164776 Copy
Species: Medicago truncatula
Genetic Insert: Medtr4g078840.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Peter/Shulan, additional dsRED selection
Proper citation: RRID:Addgene_164773 Copy
Species: Mus musculus
Genetic Insert: nAChR b2 E61C - IRES- GFP
Vector Backbone Description: Backbone Size:3728; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_164779 Copy
Species: Mus musculus
Genetic Insert: nAChR b2 E61C - IRES- GFP
Vector Backbone Description: Backbone Size:9424; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164777 Copy
Species: Medicago truncatula
Genetic Insert: Medtr4g095002.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164764 Copy
Species: Homo sapiens
Genetic Insert: p27
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16479 Copy
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