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Species: Synthetic
Genetic Insert: HyPerFLEX-UnaG
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41102408
Proper citation: RRID:Addgene_217564 Copy
Species: Synthetic
Genetic Insert: tdmito-HyPerFLEX
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41102408
Proper citation: RRID:Addgene_217567 Copy
Species: Synthetic
Genetic Insert: HyPerFLEX-Lifeact
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41102408
Proper citation: RRID:Addgene_217568 Copy
Species: Synthetic
Genetic Insert: HyPerFLEX-UnaG
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41102408
Proper citation: RRID:Addgene_217565 Copy
Species: Synthetic
Genetic Insert: TagBFP2-P2A-DAAO-NES
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41102408
Proper citation: RRID:Addgene_217566 Copy
Species: Synthetic
Genetic Insert: A-inserted T7 class III Φ6.5 promoter
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI; Vector Types:Mammalian Expression, Synthetic Biology, mRNA preparation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41102175
Comments: The A120 sequence can undergo recombination, resulting in its shortening to ~A80 or less. The likelihood of this recombination is significantly reduced when the plasmid is maintained in NEB® Stable Competent E. coli at 30 °C with 30 μg/mL kanamycin. Nevertheless, we recommend picking several colonies and verifying the poly(A) tail length. The length of the A120 sequence can be assessed through a double restriction digest using AarI and PmeI in rCutSmart™ Buffer, followed by analysis on a 3.0% agarose (1x TBE) gel. We do not recommend relying on sequencing to assess the poly(A) tail length, as long homopolymer sequences are often misread and appear shorter than they actually are.
A DNA template for in vitro transcription can be prepared from this plasmid using AarI restriction digest.
The presence of the A-inserted T7 class III Φ6.5 promoter facilitates highly efficient in vitro transcription priming with trinucleotide cap analogues such as CleanCap or other IVT primers, such as CleaN3, containing AG dinucleotide sequence that base-pairs with the promoter.
Proper citation: RRID:Addgene_225903 Copy
Species: Synthetic
Genetic Insert: A-inserted T7 class III Φ6.5 promoter
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI; Vector Types:Mammalian Expression, Synthetic Biology, mRNA preparation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41102175
Comments: The A120 sequence can undergo recombination, resulting in its shortening to ~A80 or less. The likelihood of this recombination is significantly reduced when the plasmid is maintained in NEB® Stable Competent E. coli at 30 °C with 30 μg/mL kanamycin. Nevertheless, we recommend picking several colonies and verifying the poly(A) tail length. The length of the A120 sequence can be assessed through a double restriction digest using AarI and PmeI in rCutSmart™ Buffer, followed by analysis on a 3.0% agarose (1x TBE) gel. We do not recommend relying on sequencing to assess the poly(A) tail length, as long homopolymer sequences are often misread and appear shorter than they actually are.
A DNA template for in vitro transcription can be prepared from this plasmid using AarI restriction digest.
The presence of the A-inserted T7 class III Φ6.5 promoter facilitates highly efficient in vitro transcription priming with primers, such as CleaN3, containing AG dinucleotide sequence that base-pairs with the promoter.
Proper citation: RRID:Addgene_225906 Copy
Species: Synthetic
Genetic Insert: BRD4
Vector Backbone Description: Vector Backbone:CROPseq; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34890975
Proper citation: RRID:Addgene_183331 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38409224
Proper citation: RRID:Addgene_229607 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243022 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243020 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_243024 Copy
Species: Synthetic
Genetic Insert: 12mer-FKBP
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41909827
Proper citation: RRID:Addgene_239852 Copy
Species: Synthetic
Genetic Insert: TCTP-SynPro-07::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239876 Copy
Species: Synthetic
Genetic Insert: HyPerFLEX-ERlum (Calreticulin signal sequence-HyPerFLEX-KDEL)
Vector Backbone Description: Vector Backbone:pC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41102408
Proper citation: RRID:Addgene_238941 Copy
Species: Synthetic
Genetic Insert: TCTP-SynPro-03::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239872 Copy
Species: Synthetic
Genetic Insert: TCTP-SynPro-10::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239879 Copy
Species: Synthetic
Genetic Insert: GCaMP8f
Vector Backbone Description: Backbone Marker:gift from Dr. Manuel Zimmer, University of Vienna; Backbone Size:2550; Vector Backbone:pRL231 (Punc-31_GCaMP7f_unc-54 3’UTR); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39074213
Proper citation: RRID:Addgene_239864 Copy
Species: Synthetic
Genetic Insert: CaMV 35S-SynPro-13::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239900 Copy
Species: Synthetic
Genetic Insert: CaMV 35S-SynPro-11::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239898 Copy
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