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Species: Homo sapiens
Genetic Insert: EWSR1
Vector Backbone Description: Vector Backbone:pAcGFP-N3 ; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_164701 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL1pr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164707 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tetOpr - yEVenus - CLN2 PEST - ADH1t
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: yEVenus is a codon-optimized yEGFP variant with F46L, S65G, V68L, S72A, M153T, V163A, S175G, and T203Y mutations. Please visit https://www.biorxiv.org/content/10.1101/2020.08.16.253310v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_164705 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pFLAG/HA-DGCR8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Derived from pFLAG/HA-DGCR8 made from Dr. Thomas Tuschl's lab (Addgene plasmid # 10921).
Proper citation: RRID:Addgene_164694 Copy
Species: Homo sapiens
Genetic Insert: PRMT3
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164695 Copy
Species: Homo sapiens
Genetic Insert: N-terminally truncated Drosha
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Derived from pCK-Drosha-FLAG published in Han, J., Lee, Y., Yeom, K.H., Kim, Y.K., Jin, H., and Kim, V.N. (2004). The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev 18, 3016-3027.
Proper citation: RRID:Addgene_164692 Copy
Species: Homo sapiens
Genetic Insert: N-terminally truncated transdominant negative Drosha
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Derived from pCK-DroshaTN-FLAG from Dr. V.N. Kim's lab.
Proper citation: RRID:Addgene_164693 Copy
Species: Medicago truncatula
Genetic Insert: MT35v5_AC233112_1013.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164731 Copy
Species: Homo sapiens
Genetic Insert: PRMT8
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164699 Copy
Species: Medicago truncatula
Genetic Insert: MT35v5_AC233112_1014.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164732 Copy
Species: Medicago truncatula
Genetic Insert: Medtr1g020020.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164730 Copy
Species: Medicago truncatula
Genetic Insert: Medtr7g015340.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164735 Copy
Species: Medicago truncatula
Genetic Insert: Medtr4g035905.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164736 Copy
Species: Medicago truncatula
Genetic Insert: Medtr8g446520.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164739 Copy
Species: Medicago truncatula
Genetic Insert: Medtr8g020590.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164737 Copy
Species: Medicago truncatula
Genetic Insert: Medtr6g043230.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164738 Copy
Species:
Genetic Insert: GluN2A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164724 Copy
Species:
Genetic Insert: A3A(Y130F)-zCas9(D10A)NG-UGI
Vector Backbone Description: Vector Backbone:pYPQ255; Vector Types:Plant Expression, CRISPR, Gateway compatible A3A/Y130F-nCas9-NG-CBE_V01 entry clone; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_164722 Copy
Species: Medicago truncatula
Genetic Insert: MT35v5_contig_59554_1.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:PZP200; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Peter/Shulan, additional dsRED selection
Proper citation: RRID:Addgene_164729 Copy
Species: Medicago truncatula
Genetic Insert: Medtr2g435490.1
Vector Backbone Description: Backbone Size:10475; Vector Backbone:EC50505; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned by Sonali/Shulan
Proper citation: RRID:Addgene_164726 Copy
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