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Species: Mus musculus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51584 Copy
Species: Arabidopsis thaliana
Genetic Insert: PhyB
Vector Backbone Description: Backbone Size:7236; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.
Proper citation: RRID:Addgene_51582 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51625 Copy
Species: Homo sapiens
Genetic Insert: PECAM1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 2
Proper citation: RRID:Addgene_51624 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal80
Vector Backbone Description: Vector Backbone:Gateway middle entry clone (pME); Vector Types:vertebrate expression; Bacterial Resistance:Kanamycin
References:
Comments: pME-Gal80 was cloned by PCR from TubP-GAL80 (Lee and Luo, 1999; obtained from Addgene, plasmid #17748) using primers 5'-ATGGACTACAACAAGAGATCTTCG-3' and 5'-TTATAAACTATAATGCGAGATATTGCT-3'.
Proper citation: RRID:Addgene_51620 Copy
Species: Homo sapiens
Genetic Insert: ACVR1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 1
Proper citation: RRID:Addgene_51623 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_51628 Copy
Species: Mus musculus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51595 Copy
Species: Mus musculus
Genetic Insert: shRNA1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51597 Copy
Species: Mus musculus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The Rem2 insert in this plasmid contains T13I and T15I mutations when compared to GenBank reference sequence NP_542764.2.
Proper citation: RRID:Addgene_51591 Copy
Species: Mus musculus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The Rem2 insert in this plasmid contains T13I and T15I mutations when compared to GenBank reference sequence NP_542764.2.
Proper citation: RRID:Addgene_51593 Copy
Species: Homo sapiens
Genetic Insert: Pontin
Vector Backbone Description: Backbone Marker:Invitrogen/LIfe Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51635 Copy
Species: Synthetic
Genetic Insert: Codon-optimized Cas9 D10A
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) using the primers (Forward primer, 5’-AGTACTCCATTGGGCTCGccATCGGCACAAAC, and Reverse primer, 5’-CGAGCCCAATGGAGTACTTCTTGTCGGCTGC).
For the in vitro synthesis of CAS9 D10A mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA.
The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment.
The CAG promoter in this plasmid also allows it to be used as a CAS9-expression vector.
Proper citation: RRID:Addgene_51638 Copy
Species: Homo sapiens
Genetic Insert: TERT
Vector Backbone Description: Backbone Marker:Invitrogen/LIfe Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene full plasmid sequencing noted a conserved E286D substitution in hTert.
Proper citation: RRID:Addgene_51637 Copy
Species: Mus musculus
Genetic Insert: Sema4D
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The Sema4D insert in this plasmid does not contain amino acids 1-50 and contains a L745P mutation when compared to GenBank reference sequence NP_542764.2
Proper citation: RRID:Addgene_51599 Copy
Species: Homo sapiens
Genetic Insert: ADAM10
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 6
Proper citation: RRID:Addgene_51641 Copy
Species: Homo sapiens
Genetic Insert: ACVR1B
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 5
Proper citation: RRID:Addgene_51640 Copy
Species: Homo sapiens
Genetic Insert: CD34
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 13
Proper citation: RRID:Addgene_51647 Copy
Species: Homo sapiens
Genetic Insert: CD226
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 12
Proper citation: RRID:Addgene_51646 Copy
Species: Homo sapiens
Genetic Insert: APLP2
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6618; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Article ref 7
Proper citation: RRID:Addgene_51642 Copy
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