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Species: E. coli
Genetic Insert: ACP transacylase
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_49809 Copy
Species: E. coli
Genetic Insert: αNTD
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53731 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Promega Corporation; Backbone Size:2700; Vector Backbone:pCI; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16397505
Proper citation: RRID:Addgene_44172 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Promega Corporation; Backbone Size:2700; Vector Backbone:pCI; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23319179
Proper citation: RRID:Addgene_44167 Copy
Species: E. coli
Genetic Insert: LplA(AILR) with ER-retention sequence KDEL
Vector Backbone Description: Backbone Size:4261; Vector Backbone:pCAG; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23181687
Proper citation: RRID:Addgene_42574 Copy
Species: E. coli
Genetic Insert: beta-glucoronidase (E. coli)
Vector Backbone Description: Backbone Marker:Engineering Nitrogen Symbiosis for Africa (ENSA); Backbone Size:2503; Vector Backbone:EC10161; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33072173
Proper citation: RRID:Addgene_154059 Copy
Species: E. coli
Genetic Insert: gRNA targeting pgaC
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33346713
Proper citation: RRID:Addgene_154919 Copy
Species: E. coli
Genetic Insert: gRNA targeting ampG
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33346713
Proper citation: RRID:Addgene_154923 Copy
Species: E. coli
Genetic Insert: (MlaF-6xHis)-MlaB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5531; Vector Backbone:pET21a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155144 Copy
Species: E. coli
Genetic Insert: MlaF-(MlaB-6xHis)
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155146 Copy
Species: E. coli
Genetic Insert: mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155147 Copy
Species: E. coli
Genetic Insert: MlaF-(6xHis-TEV-MlaB)
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5531; Vector Backbone:pET21a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155157 Copy
Species: E. coli
Genetic Insert: MlaF-(6xHis-TEV-MlaB)
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5531; Vector Backbone:pET21a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155158 Copy
Species: E. coli
Genetic Insert: MlaF-(6xHis-TEV-MlaB)
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5531; Vector Backbone:pET21a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155151 Copy
Species: E. coli
Genetic Insert: mlaF(1-246)-GCN4_Dimerization_Domain
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155152 Copy
Species: E. coli
Genetic Insert: MlaF-6xHis
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155150 Copy
Species: E. coli
Genetic Insert: MlaF-(6xHis-TEV-MlaB)
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5531; Vector Backbone:pET21a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32602838
Proper citation: RRID:Addgene_155161 Copy
Species: E. coli
Genetic Insert: rRNA operon, orthogonal-antiRBS
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33500394
Proper citation: RRID:Addgene_156324 Copy
Species: E. coli
Genetic Insert: EF1p_mFAP2b
Vector Backbone Description: Vector Backbone:pET15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159469 Copy
Species: E. coli
Genetic Insert: EF2n2_mFAP2b
Vector Backbone Description: Vector Backbone:pET15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159467 Copy
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