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Genetic Insert: pKW08-Lx
Vector Backbone Description: Backbone Size:7960; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20434484
Proper citation: RRID:Addgene_25012 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50367 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50370 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50371 Copy
Species: Homo sapiens
Genetic Insert: human Gamma 1 heavy chain expression cassette with unknown specificity
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50383 Copy
Species: A. victoria
Genetic Insert: Ptac::GFP-mut-2
Vector Backbone Description: Backbone Size:6050; Vector Backbone:pJN105; Vector Types:Bacterial Expression, Bacterial broad host range vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:15271914
Comments: The pBBR1MCS-5 derivative of the Bordetella bronchiseptica BHR plasmid has a pBBR/BHR replicon that is maintained in L. pneumophila and is compatible with IncP, IncW, IncQ, and IncRi incompatibility groups. Plasmid pJN105 is a derivative of pBBR1MCS-5 with araC and PBAD regions before the polylinker. PCR primers with BglII sites at their 5′ ends were used to amplify the region outside the gentamicin cassette in pJN105. The PCR fragment was digested with BglII, blunted with the Klenow fragment, and ligated to the blunted DdeI-DdeI hygromycin resistance fragment from pMV206.hygro to form pJN105-hygro. The Ptac-GFP fragment of pGS-GFP04 was amplified by PCR by using primers with NheI sites at their 5′ ends. After digestion with NheI, this fragment was ligated with NheI-digested pJN105-hygro to form pJN105-hygro-GFP.
Proper citation: RRID:Addgene_41883 Copy
Vector Backbone Description: Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33006405
Comments: Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München)
Proper citation: RRID:Addgene_42191 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61884 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Mu heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61881 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Wasabi. The Wasabi ORF is present at bp# 39-749.
Proper citation: RRID:Addgene_30174 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Teal. The Teal ORF is present at bp# 103-813.
Proper citation: RRID:Addgene_30176 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Crimson. The Crimson ORF is present at bp# 104-781.
Proper citation: RRID:Addgene_30178 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23680983
Comments: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with EBFP2. The EBFP2 ORF is present at bp# 104-823.
Proper citation: RRID:Addgene_30177 Copy
Species: M. marinum
Genetic Insert: Mycobacterium Strong Promoter (MSP)
Vector Backbone Description: Backbone Marker:Addgene Plasmid 30171; Backbone Size:0; Vector Backbone:pMSP12::dsRed2; Vector Types:Mycobacteria expression; Bacterial Resistance:Hygromycin
Comments: This plasmid was derived from pMSP12::dsRed2 by interupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.
Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#4947 when compared to the full sequence information provided by the depositing laboratory. This mismatch is not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_30170 Copy
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5304; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
This is a control plasmid for pSC301 in which the SOD promoter was excised.
Proper citation: RRID:Addgene_31852 Copy
Genetic Insert: int L5; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This expression vector integrates at the mycobacteriophage L5 site and can be stabilized by excision of the vector backbone including the int gene using the Cre recombinase; contains xylE gene as a reporter for integration; terminators to protect expression cassette against transcriptional interference.
Proper citation: RRID:Addgene_32376 Copy
Genetic Insert: gfpm2+
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Mycobacterial GFP expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18439872
Comments: This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).
Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554
Proper citation: RRID:Addgene_32362 Copy
Species: Synthetic
Genetic Insert: phGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mycobacterium tuberculosis expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18641659
Comments: The pH-GFP insert in this plasmid contains a F220L mutation compared to the wild-type pHluorin. This difference does not affect function of the pH-GFP.
Proper citation: RRID:Addgene_70045 Copy
Species: Homo sapiens
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4728; Vector Backbone:pMONOhygro-gfp (pMHG); Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:22563532
Proper citation: RRID:Addgene_72850 Copy
Species: Homo sapiens
Genetic Insert: Human immunoglobulin gamma 1 constant region
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_52213 Copy
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