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Species: E. coli
Genetic Insert: gfp-pdt#4
Vector Backbone Description: Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75457 Copy
Species: E. coli
Genetic Insert: pdt1E
Vector Backbone Description: Vector Backbone:pWM91; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75449 Copy
Species: E. coli
Genetic Insert: pdt1
Vector Backbone Description: Vector Backbone:pWM91; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75446 Copy
Species: E. coli
Genetic Insert: Thr251Gly-EcPheRS
Vector Backbone Description: Backbone Marker:Beckwith Lab; Backbone Size:5319; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_72669 Copy
Species: E. coli
Genetic Insert: AlkB
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5310; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17213319
Proper citation: RRID:Addgene_73622 Copy
Species: E. coli
Genetic Insert: tra Operon (second Quarter)
Vector Backbone Description: Backbone Marker:Registry of Standard Parts; Vector Backbone:pSB1A3; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_67969 Copy
Species: E. coli
Genetic Insert: TraM, TraJ
Vector Backbone Description: Backbone Marker:Registry of Standard Parts; Vector Backbone:pSB3C5; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_67966 Copy
Species: E. coli
Genetic Insert: aac3(IV)
Vector Backbone Description: Vector Backbone:pSET152; Vector Types:Streptomyces integrating vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:12923110
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_68110 Copy
Species: E. coli
Genetic Insert: aac3(IV)
Vector Backbone Description: Vector Backbone:pSET152; Vector Types:Streptomyces integrating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12923110
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_68113 Copy
Species: E. coli
Genetic Insert: Coding sequence, hygromycin phophotransferase II (Escherichia coli)
Vector Backbone Description: Vector Backbone:pICH41308 AddGene #47998; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26616834
Proper citation: RRID:Addgene_68259 Copy
Species: E. coli
Genetic Insert: Coding sequence, neomycin phophotransferase II (Escherichia coli)
Vector Backbone Description: Vector Backbone:pICH41308 AddGene #47998; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26616834
Proper citation: RRID:Addgene_68260 Copy
Species: E. coli
Genetic Insert: pstS (A197C)
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199994
Comments: Please note- The numbering system used was for the mature natural wild-type PstS protein. The plasmid produces an extra N-terminal methionine so all mutations will be off by 1nt (e.g., A197C =A198C)
The expressed protein is a single-cysteine variant of E. coli phosphate binding protein. The gene encodes the mature protein without the N-terminal signal peptide, but with an N-terminal methionine for subsequent labeling at the cysteine with one MDCC (N-[2-(1-maleimidyl)ethyl]-7-diethylaminocoumarin-3-carboxamide) per molecule. This adduct is then used as a fluorescent phosphate biosensor, having ~7-fold fluorescence intensity increase on binding inorganic phosphate with very high affinity.
Proper citation: RRID:Addgene_78198 Copy
Species: E. coli
Genetic Insert: bla
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31604953
Comments: This plasmid is a derivative of repressilator plasmids presented in: Potvin-Trottier et al, 2016, Nature.
Proper citation: RRID:Addgene_127855 Copy
Species: E. coli
Genetic Insert: Maltose binding protein
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Comments: Depositor confirms mutation Y342H does not affect plasmid function.
Proper citation: RRID:Addgene_128398 Copy
Species: E. coli
Genetic Insert: Maltose binding protein
Vector Backbone Description: Backbone Size:1765; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33536221
Comments: Depositor confirms mutation does not affect plasmid function.
Proper citation: RRID:Addgene_128390 Copy
Species: E. coli
Genetic Insert: Flagellin
Vector Backbone Description: Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33381753
Proper citation: RRID:Addgene_128853 Copy
Species: E. coli
Genetic Insert: Flagellin
Vector Backbone Description: Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33381753
Proper citation: RRID:Addgene_128851 Copy
Species: E. coli
Genetic Insert: Flagellin
Vector Backbone Description: Backbone Marker:ATUM; Backbone Size:4000; Vector Backbone:pJ291; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33381753
Proper citation: RRID:Addgene_128856 Copy
Species: E. coli
Genetic Insert: Flagellin
Vector Backbone Description: Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33381753
Proper citation: RRID:Addgene_128849 Copy
Species: E. coli
Genetic Insert: Flagellin
Vector Backbone Description: Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33381753
Proper citation: RRID:Addgene_128848 Copy
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