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Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::mCherry (w introns w/o stop)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2646; Vector Backbone:pDONRP4P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_22513 Copy
Species: Caenorhabditis elegans
Genetic Insert: SnAvi
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9674; Vector Backbone:EGFP-N1; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20047968
Comments: this plasmid encodes the new described SnAvi tag and additionally the unc-119 rescue sequence from C. elegans (bp4-5705)
Proper citation: RRID:Addgene_23223 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26387 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::GFP::unc-54_3'UTR
Vector Backbone Description: Backbone Size:10813; Vector Backbone:pBCN27-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729840
Comments: The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected.
Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.
Proper citation: RRID:Addgene_26347 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7 hairpin only
Vector Backbone Description: Backbone Size:6946; Vector Backbone:MDH1-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26488 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7 (~250nt flanking each side)
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7060; Vector Backbone:MXW-PGK-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: Ordinarily, we express miRNAs from the LTR, which is pol III. However, cel-let-7 won't express from that promoter (possibly due to long poly-T tracts). This is a pol II expression system.
Proper citation: RRID:Addgene_26491 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPB
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS. Used for in vitro translation.
Proper citation: RRID:Addgene_26560 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPA
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS. Used for in vitro translation.
Proper citation: RRID:Addgene_26559 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS.
Loop mutants have also all been cloned into this backbone for in vitro translation.
Proper citation: RRID:Addgene_26558 Copy
Species: Caenorhabditis elegans
Genetic Insert: Unc104
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12351789
Proper citation: RRID:Addgene_24476 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-12(P687S)
Vector Backbone Description: Vector Backbone:pCMV myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21814518
Proper citation: RRID:Addgene_32567 Copy
Species: Caenorhabditis elegans
Genetic Insert: T04C9.2 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33198 Copy
Species: Caenorhabditis elegans
Genetic Insert: T20G5.9 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33193 Copy
Species: Caenorhabditis elegans
Genetic Insert: glb-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33190 Copy
Species: Caenorhabditis elegans
Genetic Insert: T04C9.2 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33197 Copy
Species: Caenorhabditis elegans
Genetic Insert: acl-5 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33195 Copy
Species: Caenorhabditis elegans
Genetic Insert: glb-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33189 Copy
Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33220 Copy
Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33221 Copy
Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33226 Copy
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