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Genetic Insert: tetR(sB), clpXtb
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_27373 Copy
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5304; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
This is a control plasmid for pSC301 in which the SOD promoter was excised.
Proper citation: RRID:Addgene_31852 Copy
Genetic Insert: int L5; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This expression vector integrates at the mycobacteriophage L5 site and can be stabilized by excision of the vector backbone including the int gene using the Cre recombinase; contains xylE gene as a reporter for integration; terminators to protect expression cassette against transcriptional interference.
Proper citation: RRID:Addgene_32376 Copy
Genetic Insert: gfpm2+
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Mycobacterial GFP expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18439872
Comments: This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).
Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554
Proper citation: RRID:Addgene_32362 Copy
Species: A. victoria
Genetic Insert: Ptac::GFP-mut-2
Vector Backbone Description: Backbone Size:6050; Vector Backbone:pJN105; Vector Types:Bacterial Expression, Bacterial broad host range vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:15271914
Comments: The pBBR1MCS-5 derivative of the Bordetella bronchiseptica BHR plasmid has a pBBR/BHR replicon that is maintained in L. pneumophila and is compatible with IncP, IncW, IncQ, and IncRi incompatibility groups. Plasmid pJN105 is a derivative of pBBR1MCS-5 with araC and PBAD regions before the polylinker. PCR primers with BglII sites at their 5′ ends were used to amplify the region outside the gentamicin cassette in pJN105. The PCR fragment was digested with BglII, blunted with the Klenow fragment, and ligated to the blunted DdeI-DdeI hygromycin resistance fragment from pMV206.hygro to form pJN105-hygro. The Ptac-GFP fragment of pGS-GFP04 was amplified by PCR by using primers with NheI sites at their 5′ ends. After digestion with NheI, this fragment was ligated with NheI-digested pJN105-hygro to form pJN105-hygro-GFP.
Proper citation: RRID:Addgene_41883 Copy
Vector Backbone Description: Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33006405
Comments: Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München)
Proper citation: RRID:Addgene_42191 Copy
Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:4921; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21209917
Comments: Plasmid contains 2 His tags within the multiple cloning site. Please see plasmid map from depositing laboratory above for details.
Proper citation: RRID:Addgene_37277 Copy
Species: Synthetic
Genetic Insert: Fab_Theo-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119048 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119884 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117232 Copy
Species: M. smegmatis
Genetic Insert: gfp, hyg
Vector Backbone Description: Vector Backbone:pYS2; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24100088
Comments: Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117490 Copy
Species: Other
Genetic Insert: Htb2-GFP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30314480
Comments: Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_117669 Copy
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AB; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30698703
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117827 Copy
Species: A. niger
Genetic Insert: gRNA1 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118078 Copy
Vector Backbone Description: Backbone Marker:Matthias Steiger Martin Altvater; Backbone Size:2852; Vector Backbone:MST607; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118077 Copy
Species: A. niger
Genetic Insert: gRNA2 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118079 Copy
Species: A. niger
Genetic Insert: pTPI1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118075 Copy
Species: Homo sapiens
Genetic Insert: PIK3C3 and PIK3R4
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6321; Vector Backbone:pVitro2; Vector Types:Mammalian Expression, bicistronic; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18957027
Proper citation: RRID:Addgene_24055 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24659 Copy
Species: Synthetic gene
Genetic Insert: Turbo
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24658 Copy
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