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Species: Mus musculus
Genetic Insert: Fgfr2 donor region including homology arms and T2A-H2B-Halo
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113117 Copy
Species: Mus musculus
Genetic Insert: pgk-1 5' and 3' regions
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3440520
Comments: Includes 500 bp mouse PGK promoter, 832 bp neo, 460 bp PGK 3' end.
Proper citation: RRID:Addgene_11333 Copy
Species: Mus musculus
Genetic Insert: mAID-BE3
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113420 Copy
Species: Mus musculus
Genetic Insert: mA1-BE3
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113421 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113501 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113504 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113503 Copy
Species: Mus musculus
Genetic Insert: p37
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113502 Copy
Species: Mus musculus
Genetic Insert: PAR2
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29411438
Proper citation: RRID:Addgene_113609 Copy
Species: Mus musculus
Genetic Insert: I-Ab beta Derp1-117
Vector Backbone Description: Backbone Size:3818; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding alpha chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes. The antigenic peptide sequence (residues 117-127 of Der p 1 allergen) is encoded as a fusion to the N-terminus of I-Ab beta, connected via a Gly-Ser linker. This fusion is encoded just downstream of the signal peptide, which should be cleaved during post-translational processing. Alternative antigenic peptides can be cloned in place of Der p 1 117-127 by exploiting the XmaI and SpeI restriction sites flanking this sequence.
Proper citation: RRID:Addgene_113639 Copy
Species: Mus musculus
Genetic Insert: I-Ab alpha BirA
Vector Backbone Description: Backbone Size:3808; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding beta chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes.
Proper citation: RRID:Addgene_113638 Copy
Species: Mus musculus
Genetic Insert: miR-223
Vector Backbone Description: Backbone Size:6963; Vector Backbone:MDH1-PGK-GFP 2.0; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14657504
Proper citation: RRID:Addgene_11378 Copy
Species: Mus musculus
Genetic Insert: the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423323
Proper citation: RRID:Addgene_114130 Copy
Species: Mus musculus
Genetic Insert: the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423323
Proper citation: RRID:Addgene_114135 Copy
Species: Mus musculus
Genetic Insert: the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423323
Proper citation: RRID:Addgene_114134 Copy
Species: Mus musculus
Genetic Insert: 18747
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423323
Proper citation: RRID:Addgene_114132 Copy
Species: Mus musculus
Genetic Insert: Enhanced Green Fluorescent Protein
Vector Backbone Description: Vector Backbone:pHR (lentivirus); Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996091
Proper citation: RRID:Addgene_114212 Copy
Species: Mus musculus
Genetic Insert: Enhanced Green Fluorescent Protein
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996091
Comments: Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied sequence. The authors have noted that the discrepancies do NOT affect plasmid function or viral packaging.
Proper citation: RRID:Addgene_114214 Copy
Species: Mus musculus
Genetic Insert: Itch
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5400; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12907674
Comments: Plasmid contains ~1kb of 3' UTR of the mouse Itch gene.
Proper citation: RRID:Addgene_11427 Copy
Species: Mus musculus
Genetic Insert: Nedd4-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12907674
Proper citation: RRID:Addgene_11426 Copy
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