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Genetic Insert: int L5; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This expression vector integrates at the mycobacteriophage L5 site and can be stabilized by excision of the vector backbone including the int gene using the Cre recombinase; contains xylE gene as a reporter for integration; terminators to protect expression cassette against transcriptional interference.
Proper citation: RRID:Addgene_32376 Copy
Genetic Insert: gfpm2+
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Mycobacterial GFP expression vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18439872
Comments: This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).
Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554
Proper citation: RRID:Addgene_32362 Copy
Species: Homo sapiens
Genetic Insert: human Gamma 1 heavy chain expression cassette with unknown specificity
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50383 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61884 Copy
Species: Homo sapiens
Genetic Insert: HER2/neu receptor specific humanized Mu heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_61881 Copy
Species: Homo sapiens
Genetic Insert: Human immunoglobulin gamma 1 constant region
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_52213 Copy
Species: Homo sapiens
Genetic Insert: Human immunoglobulin gamma 1 constant region
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_52214 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50367 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50370 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:25073855
Proper citation: RRID:Addgene_50371 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:26934495
Proper citation: RRID:Addgene_157877 Copy
Species: Other
Genetic Insert: crRNA
Vector Backbone Description: Backbone Marker:from Houhui Song's Lab; Vector Backbone:pSL001; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology, Mycobacteria Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28646112
Comments: Please note that several differences were identified in the plasmid backbone compared to the depositor's sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_158708 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158721 Copy
Species: Acidaminococcus sp. BV3L6
Genetic Insert: Ascpf1
Vector Backbone Description: Vector Backbone:pSIM18; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33710766
Proper citation: RRID:Addgene_153034 Copy
Species: Synthetic
Genetic Insert: CymR(AM)-mRFP
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34125913
Proper citation: RRID:Addgene_149494 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149559 Copy
Species: Synthetic
Genetic Insert: sgRNAftsI
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149563 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(RBSmut); Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149580 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149573 Copy
Genetic Insert: n/a
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pSMT3; Vector Types:Shuttle vector for mycobacteria; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Comments: Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb) 87: 481–488.
There are known discrepancies between the Addgene QC sequence and the depositor's full sequence--these are likely due to how the full sequence was assembled and should not affect function.
Proper citation: RRID:Addgene_26589 Copy
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