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Species: Drosophila melanogaster
Genetic Insert: Torso
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-7Zf; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9892666
Comments: Perrimon Lab plasmid #215
Proper citation: RRID:Addgene_39797 Copy
Species: Drosophila melanogaster
Genetic Insert: CG18023
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_39719 Copy
Species: Drosophila melanogaster
Genetic Insert: CG8643
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_39718 Copy
Species: Drosophila melanogaster
Genetic Insert: CG6794
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_39704 Copy
Species: Drosophila melanogaster
Genetic Insert: CG10571
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2536; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22037703
Comments: Please note that the ORFs in this collection were cloned open-ended (without a stop codon).
Proper citation: RRID:Addgene_39709 Copy
Species: Drosophila melanogaster
Genetic Insert: Torso genomic DNA fragment
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2464; Vector Backbone:pSP73; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9892666
Comments: Perrimon Lab plasmid #228
Proper citation: RRID:Addgene_39810 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm14-21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 mm14-21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATAGTCCGTACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTACGGACTAT
Proper citation: RRID:Addgene_40851 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm15-21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 mm15-21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATAACCCGAACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTTCGGGTTAT
Proper citation: RRID:Addgene_40850 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm1-8
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 mm1-8 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGAACTATACAACCTAGATGGAGTT
as: CTAGAACTCCATCTAGGTTGTATAGTT
Proper citation: RRID:Addgene_40847 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–bulge9-15
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 bulge9-15 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGAACTATAGTTGGATCTACCTCAT
as: CTAGATGAGGTAGATCCAACTATAGTT
Proper citation: RRID:Addgene_40846 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–bulge 9-13
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 bulge 9-13 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGAACTATACATGGATCTACCTCAT
as: CTAGATGAGGTAGATCCATGTATAGTT
Proper citation: RRID:Addgene_40845 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–seed1-8
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 seed1-8 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATAACACGTTGGCTCTACCTCAT
as: CTAGATGAGGTAGAGCCAACGTGTTAT
Proper citation: RRID:Addgene_40843 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm16-21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 mm16-21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATGACACCAACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTTGGTGTCAT
Proper citation: RRID:Addgene_40849 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–Bartel
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 Bartel complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATGATAACAACGATCTACCTCAT
as: CTAGATGAGGTAGATCGTTGTTATCAT
Proper citation: RRID:Addgene_40848 Copy
Species: Drosophila melanogaster
Genetic Insert: Four target sites perfectly complementary to let-7
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20558712
Comments: The let-7 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTA GAA CTA TAC AAC CTA CTA CCT CAT CTA GAA CTA TAC AAC CTA CTA CCT CAT
as: CTA GAT GAG GTA GTA GGT TGT ATA GTT CTA GAT GAG GTA GTA GGT TGT ATA GTT
This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
Proper citation: RRID:Addgene_40834 Copy
Species: Drosophila melanogaster
Genetic Insert: Two target sites perfectly complementary to miR-1
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20558712
Comments: The miR-1 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTA GAC TCC ATA CTT CTT TAC ATT CCA TCT AGA CTC CAT ACT TCT TTA CAT TCC AT
as: CTA GAT GGA ATG TAA AGA AGT ATG GAG TCT AGA TGG AAT GTA AAG AAG TAT GGA GT
This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
Proper citation: RRID:Addgene_40832 Copy
Species: Drosophila melanogaster
Genetic Insert: three sites partially complementary to miR-34
Vector Backbone Description: Backbone Marker:promega; Backbone Size:6273; Vector Backbone:psiCheck-2; Vector Types:Insect Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22055293
Comments: The psiCheck-3xmiR-34 sequences were cloned by synthesized dsDNA
S: 5'-TCGAGGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGC
AS: 5'-GGCCGCTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACC
the triple(3x)-repeated sequence:
5'TGGCAGTGACCTTAGCATCAACAC-3'
3'ACCGTCACTGGAATCGTAGTTGTG-5'
pairs with dme-miR-34 (uggcagugugguuagcugguugug) with "seed(red) plus 3' complementary region (blue)" fashion.
Proper citation: RRID:Addgene_40831 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm19-21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7–mm19-21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATGAATACAACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTTGTATTCAT
Proper citation: RRID:Addgene_40839 Copy
Species: Drosophila melanogaster
Genetic Insert: let-7–mm21
Vector Backbone Description: Backbone Marker:Zamore lab; Vector Backbone:pENTR/D-EGFP; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20558712
Comments: The let-7 mm21 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos:
s: CTAGATCTATACAACCTACTACCTCAT
as: CTAGATGAGGTAGTAGGTTGTATAGAT
Proper citation: RRID:Addgene_40837 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab39
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17409086
Proper citation: RRID:Addgene_37745 Copy
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