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Species: Synthetic
Genetic Insert: NG NN NG
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63543 Copy
Species: Synthetic
Genetic Insert: NG NG NG
Vector Backbone Description: Backbone Size:3008; Vector Backbone:FusX4; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63552 Copy
Species: Synthetic
Genetic Insert: NG NG HD
Vector Backbone Description: Backbone Size:3008; Vector Backbone:FusX4; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63550 Copy
Species: Synthetic
Genetic Insert: HD HD NG
Vector Backbone Description: Backbone Size:2991; Vector Backbone:FusB3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26854857
Proper citation: RRID:Addgene_63509 Copy
Vector Backbone Description: Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25855067
Proper citation: RRID:Addgene_64249 Copy
Vector Backbone Description: Backbone Marker:Adam Bogdanove, Daniel Voytas lab; Backbone Size:2568; Vector Backbone:pLR-NN; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25855067
Proper citation: RRID:Addgene_64248 Copy
Genetic Insert: GUS
Vector Backbone Description: Vector Backbone:pGreenIIS BAR; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25448000
Proper citation: RRID:Addgene_64822 Copy
Species: Synthetic
Genetic Insert: SpecR-ColE1
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65192 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58779 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58781 Copy
Species: S. pyogenes
Genetic Insert: humanized S. pyogenes Cas9 nuclease
Vector Backbone Description: Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24954249
Proper citation: RRID:Addgene_58782 Copy
Species: Synthetic
Genetic Insert: Three-fragment T7* RNA polymerase
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59952 Copy
Vector Backbone Description: Backbone Marker:Haldimann,A. and Wanner,B.L.; Backbone Size:2000; Vector Backbone:pINTts; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:9843682
Proper citation: RRID:Addgene_59802 Copy
Species: Synthetic
Genetic Insert: T3 sigma fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59955 Copy
Species: Synthetic
Genetic Insert: T7 sigma fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59954 Copy
Species: Synthetic
Genetic Insert: CGG sigma fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59957 Copy
Species: Synthetic
Genetic Insert: alpha fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59959 Copy
Species: Synthetic
Genetic Insert: null fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59958 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23836671
Proper citation: RRID:Addgene_60533 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23836671
Comments: This plasmid is derived from Addgene plasmid #23850. We changed a1208c, g79c to align with pubmed sequence and added a stop codon.
Proper citation: RRID:Addgene_60532 Copy
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