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http://www.addgene.org/30093

Species: E. coli
Genetic Insert: pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_30093 Copy   

•   Source: Addgene

http://www.addgene.org/30094

Species: E. coli
Genetic Insert: pLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_30094 Copy   

•   Source: Addgene

http://www.addgene.org/30086

Species: E. coli
Genetic Insert: pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_30086 Copy   

•   Source: Addgene

http://www.addgene.org/30088

Species: E. coli
Genetic Insert: pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

Proper citation: RRID:Addgene_30088 Copy   

•   Source: Addgene

http://www.addgene.org/31172

Species: E. coli
Genetic Insert: E. coli elongation factor Tu
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8765000

Proper citation: RRID:Addgene_31172 Copy   

•   Source: Addgene

http://www.addgene.org/31340

Species: E. coli
Genetic Insert: E. coli Initiation Factor 3
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET21c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12095986

Proper citation: RRID:Addgene_31340 Copy   

•   Source: Addgene

http://www.addgene.org/31398

Species: E. coli
Genetic Insert: zapB
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18394147

Proper citation: RRID:Addgene_31398 Copy   

•   Source: Addgene

http://www.addgene.org/35041

Species: e. coli
Genetic Insert: cytochrome b562
Vector Backbone Description: Vector Backbone:pUC 18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1761034

Proper citation: RRID:Addgene_35041 Copy   

•   Source: Addgene

http://www.addgene.org/35948

Species: E. Coli
Genetic Insert: Subtilase A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21857923

Proper citation: RRID:Addgene_35948 Copy   

•   Source: Addgene

http://www.addgene.org/36256

Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Stover et al., 1991 Nature 351:456; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacteria replicating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22279533
Comments: The plasmid is high copy in E. coli, but low copy in mycobacteria. Cloning was done via assembly PCR, sot he XbaI-HindIII fragment comprises the promoter-riboswitch. The insert is 3361bp including the promoter. There are mismatches between Addgene's quality control sequence and the depositor sequence, but they are not known to affect plasmid function.

Proper citation: RRID:Addgene_36256 Copy   

•   Source: Addgene

http://www.addgene.org/33144

Species: E. coli
Genetic Insert: LacO (256 copies)
Vector Backbone Description: Backbone Marker:Addgene plasmid #33143; Vector Backbone:pVS1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18614049
Comments: Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 [Addgene #33143] is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.

Proper citation: RRID:Addgene_33144 Copy   

•   Source: Addgene

http://www.addgene.org/33259

Species: E. coli
Genetic Insert: LexA::VP16-SV40 3'UTR
Vector Backbone Description: Vector Backbone:pCasPeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19060186
Comments: A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006). The PCR fragment was then subcloned into the XbaI site of the pBS vector. LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter.

Proper citation: RRID:Addgene_33259 Copy   

•   Source: Addgene

http://www.addgene.org/33376

Species: E. coli
Genetic Insert: phnD based Phosphonate Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22019591
Comments: The EcPhnD-cpGFP insertion variants were constructed by overlap PCR using the synthetic EcPhnD sequence and the cpGFP146 variant from GCaMP2. Detailed sequences are provided in attached sheets. The linker regions were mutated by single-stranded template mutagenesis

Proper citation: RRID:Addgene_33376 Copy   

•   Source: Addgene

http://www.addgene.org/33372

Species: E. coli
Genetic Insert: MBP based Maltose Biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21989929
Comments: The MBP-cpGFP insertion variant was constructed by overlap PCR using the wild-type MBP sequence from the pMalE-p4E vector (New England Biolabs) and the cpGFP146 variant from GCaMP2 into pRSET-A between BamHI and EcoRI sites. The N-terminal periplasmic leader sequence of MBP was excluded, and a BamHI site (encoding Gly-Ser) was included at the 5′-end. At the C-terminus, an additional His6Gly tag was included after the terminus of MBP (a cloning oversight). An EcoRI site was included after the stop codon.

Proper citation: RRID:Addgene_33372 Copy   

•   Source: Addgene

http://www.addgene.org/34627

Species: E. coli
Genetic Insert: EF-Sep
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21868676
Comments: To construct pMAL-EFSep the gene encoding EF-Sep, was cloned between the NdeI and BamHI sites in the pET20b plasmid (Novaven) to add a C-terminal His6 tag. This fusion construct was then PCR-amplified using primers adding MfeI and PstI restriction sites. The PCR product was cloned in-frame between EcoRI and PstI in pMALc2x (New England Biolabs) to add an N-terminal maltose binding protein (MBP) tag. To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.

Proper citation: RRID:Addgene_34627 Copy   

•   Source: Addgene

http://www.addgene.org/28029

Species: E. coli
Genetic Insert: MutS
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9308969
Comments: pSL6 contains a ClaI–BamHI fragment of pMS312 carrying E. coli mutS and the KanR-conferring HindIII–BamHI fragment of pKC31

Proper citation: RRID:Addgene_28029 Copy   

•   Source: Addgene

http://www.addgene.org/28000

Species: E. Coli
Genetic Insert: FLIPglu-2µΔ13V
Vector Backbone Description: Backbone Size:6866; Vector Backbone:pDRf1GW-ura3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20854260

Proper citation: RRID:Addgene_28000 Copy   

•   Source: Addgene

http://www.addgene.org/29326

Species: E. coli
Genetic Insert: YFP-DHFR(DD)
Vector Backbone Description: Backbone Marker:Garry Nolan; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20851347

Proper citation: RRID:Addgene_29326 Copy   

•   Source: Addgene

http://www.addgene.org/111183

Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9354; Vector Backbone:pLXI_TRC403; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30860482

Proper citation: RRID:Addgene_111183 Copy   

•   Source: Addgene

http://www.addgene.org/111184

Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:Broad Institute; Backbone Size:9396; Vector Backbone:pLXI_TRC403; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30860482

Proper citation: RRID:Addgene_111184 Copy   

•   Source: Addgene