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Species: Synthetic
Genetic Insert: yoTagRFP-T
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23844123
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
These plasmids were generated with funding from an NIGMS Systems Biology Center grant, number P50 GM081879. Please acknowledge this grant if you use these plasmids.
Proper citation: RRID:Addgene_44842 Copy
Genetic Insert: hhf2
Vector Backbone Description: Vector Backbone:pGF1 1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7729422
Proper citation: RRID:Addgene_44959 Copy
Species: Synthetic
Genetic Insert: yomKO2
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23844123
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
These plasmids were generated with funding from an NIGMS Systems Biology Center grant, number P50 GM081879. Please acknowledge this grant if you use these plasmids.
Proper citation: RRID:Addgene_44958 Copy
Species: Synthetic
Genetic Insert: DnvLB16
Vector Backbone Description: Backbone Size:6287; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23827138
Proper citation: RRID:Addgene_45124 Copy
Species: Synthetic
Genetic Insert: yoClover
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23844123
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
These plasmids were generated with funding from an NIGMS Systems Biology Center grant, number P50 GM081879. Please acknowledge this grant if you use these plasmids.
Proper citation: RRID:Addgene_44952 Copy
Species: Schmidtea mediterranea
Genetic Insert: Stem Loop Binding Protein
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:RNAi, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24903754
Proper citation: RRID:Addgene_45082 Copy
Species: Schmidtea mediterranea
Genetic Insert: Histone H3
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:RNAi, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24903754
Proper citation: RRID:Addgene_45086 Copy
Species: Schmidtea mediterranea
Genetic Insert: Histone H2B
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:RNAi, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24903754
Proper citation: RRID:Addgene_45085 Copy
Species: Synthetic
Genetic Insert: HtsptLB12v.3
Vector Backbone Description: Backbone Size:6287; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23827138
Proper citation: RRID:Addgene_45121 Copy
Species: Schmidtea mediterranea
Genetic Insert: Histone H3 imperfect stem
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:3000; Vector Backbone:pBluescript II SK(+); Vector Types:RNAi, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24903754
Proper citation: RRID:Addgene_45087 Copy
Species: Synthetic
Genetic Insert: HtsptLB12
Vector Backbone Description: Backbone Size:6287; Vector Backbone:pETCON; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23827138
Proper citation: RRID:Addgene_45120 Copy
Species: Mus musculus
Genetic Insert: alpha4-mcherry
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21187334
Comments: Principal Investigator Henry A. Lester
α4 cloned into EcoRI sites of pCI-neo
Proper citation: RRID:Addgene_45073 Copy
Species: Homo sapiens
Genetic Insert: Lacritin
Vector Backbone Description: Backbone Marker:New England Biolabs, INC; Backbone Size:7477; Vector Backbone:pTYB1; Vector Types:Bacterial Expression, Protein Expression Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16923831
Comments: Please note that Addgene's sequencing results identified a few differences in the vector backbone when compared to the full plasmid sequence. The depositing laboratory indicates that these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_45072 Copy
Species: Homo sapiens
Genetic Insert: CENP-T (aa1-374) Laci-NLS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pIC242; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21529714
Proper citation: RRID:Addgene_45109 Copy
Species: Synthetic
Genetic Insert: cAMP-dependent protein kinase inhibitor alpha
Vector Backbone Description: Backbone Marker:Maurer lab; Backbone Size:4265; Vector Backbone:pRSV-link; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Derived from RSV-PKI constructed by the Maurer lab and described in Day et al., J. Biol. Chem. 264, 431-436, 1989. Altered to add a Kozak consensus translation initiation sequence and also the ori was changed to yield high copy number in E coli.
Proper citation: RRID:Addgene_45067 Copy
Species: Homo sapiens
Genetic Insert: TfR
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839364
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_45060 Copy
Species: Gallus gallus
Genetic Insert: NgCam
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839364
Comments: NgCam is very GC rich and difficult to sequence and PCR.
pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Addgene's sequencing results found a few nucleotide differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid.
Proper citation: RRID:Addgene_45061 Copy
Species: Rattus norvegicus
Genetic Insert: CaM Kinase II
Vector Backbone Description: Backbone Marker:Maurer lab; Backbone Size:4265; Vector Backbone:pRSV-link; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Proper citation: RRID:Addgene_45064 Copy
Species: Mus musculus
Genetic Insert: Cam Kinase IV(1-313)
Vector Backbone Description: Backbone Marker:Maurer lab; Backbone Size:4265; Vector Backbone:pRSV-link; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Comments: This CaMKIV has a R128A mutation. The depositor considers this a polymorphism that is not functionally relevant.
Proper citation: RRID:Addgene_45063 Copy
Species: Rattus norvegicus
Genetic Insert: Kif5c
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16543128
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_45059 Copy
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