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  • Organism:severe acute respiratory syndrome coronavirus 2 (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pGBW-m4133582
 
Resource Report
Resource Website
RRID:Addgene_154802 SARS-CoV-2 ORF6 protein Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI reference sequence: YP_009724394.1 Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin H. sapiens recode 1 2026-08-15 01:07:54 0
HDM-SARS2-Spike-delta21
 
Resource Report
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10+ mentions
RRID:Addgene_155130 SARS-CoV-2 Spike-delta21 Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:32384820 Based on a plasmid described previously in Crawford et al. 2020, Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays (https://www.mdpi.com/1999-4915/12/5/513). This is a version of the Spike expression plasmid used to pseudotype lentivirus which provided better titers in this protocol. Backbone Size:4750; Vector Backbone:HDM; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Codon optimized to H. sapiens using IDT codon optimization tool; deleted 21 amino acids by deletion from 3769-3831 2026-08-15 01:07:59 20
pGBW-m4046471
 
Resource Report
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RRID:Addgene_145398 SARS-CoV-2 endoRNAse Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:55 0
pGBW-m4046266
 
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Resource Website
RRID:Addgene_145394 SARS-CoV-2 nsp7 Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI Reference Sequence: YP_009725303.1. Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:55 0
pGBW-m4046517
 
Resource Report
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RRID:Addgene_145392 SARS-CoV-2 nsp11 Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI Reference Sequence: YP_009725312.1. Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Saccharomyces cerevisiae recode 1 2026-08-15 01:06:55 0
pGBW-m4046257
 
Resource Report
Resource Website
RRID:Addgene_145393 SARS-CoV-2 nsp8 Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI Reference Sequence: YP_009725304.1. Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:55 0
pGBW-m4046230
 
Resource Report
Resource Website
RRID:Addgene_145454 SARS-CoV-2 ORF3a protein Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI Reference Sequence: YP_009724391.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:56 0
pGBW-m4046463
 
Resource Report
Resource Website
RRID:Addgene_145462 SARS-CoV-2 nsp10 Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI Reference Sequence: YP_009725306.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:56 0
pGBW-m4046273
 
Resource Report
Resource Website
RRID:Addgene_145480 SARS-CoV-2 ORF10 protein Severe acute respiratory syndrome coronavirus 2 Ampicillin NCBI Reference Sequence: YP_009725255.1Created by gene synthesis with Not1 site. Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin wt 2026-08-15 01:06:56 0
Spike Display_B.1.351
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_172736 SARS-CoV-2 S B.1.351 Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34919820 Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint. Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P, D614G, L18F, D80A, D215G, 242-244 Deletion, R246I, K417N, E484K, N501Y, A701V 2026-08-15 01:10:27 1
Spike Display_Part 5 Spacer
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_172733 SARS-CoV-2 S HexaPro-D614G Severe acute respiratory syndrome coronavirus 2 Chloramphenicol PMID:34919820 Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint. Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol Ectodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P, D614G 2026-08-15 01:10:27 1
Spike Display_B.1.427/B.1.429
 
Resource Report
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1+ mentions
RRID:Addgene_172738 SARS-CoV-2 S B.1.427/B.1.429 Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34919820 Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint. Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P, D614G, S13I, W152C, L452R 2026-08-15 01:10:27 1
Spike Display_Part-5 DO
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_172725 SARS-CoV-2 S HexaPro-D614G Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34919820 Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint. Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P, D614G 2026-08-15 01:10:27 1
Spike Display_Part 3 DO
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_172723 SARS-CoV-2 S HexaPro-D614G Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34919820 Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint. Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P, D614G 2026-08-15 01:10:27 1
pαH-S-GSAS-B.1.351.v3
 
Resource Report
Resource Website
RRID:Addgene_173787 Spike (S-GSAS-B.1.351 variant version 3) Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34168071 Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; D614G; N501Y; K417N; E484K; L18F; D80A; D215G; R246I; A701V;L242H 2026-08-15 01:10:34 0
pαH-S-GSAS-B.1.351.v4
 
Resource Report
Resource Website
RRID:Addgene_173788 Spike (S-GSAS-B.1.351 variant version 4) Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34168071 Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; D614G; N501Y; K417N; E484K; L18F; D80A; D215G; A701V;Del242/244 2026-08-15 01:10:34 0
pαH-S-GSAS-P1-like
 
Resource Report
Resource Website
RRID:Addgene_173789 Spike S-GSAS- P-1-like variant (K417N) Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34168071 Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; D614G; N501Y; K417N; E484K; L18F; T20N;P26S; D138Y; R190S;-H655Y;T1027I 2026-08-15 01:10:34 0
pTwist-SARS-CoV-2 Δ18 B.1.617.2v1
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_179905 SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34995482 Backbone Marker:Twist; Backbone Size:6694; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G; P681R; D950N; 2026-08-15 01:11:23 6
pTwist-SARS-CoV-2 Δ18 B.1.1.529
 
Resource Report
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10+ mentions
RRID:Addgene_179907 SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids Severe acute respiratory syndrome coronavirus 2 Ampicillin PMID:34995482 Balazs lab Clone 18 Backbone Marker:Twist; Backbone Size:6694; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212; Ins214EPE; G339D; S371L; S373P; S375F; K417N; N440K; G446S; S477N; T478K; E484A; Q493R; G496S; Q498R; N501Y; Y505H; T547K; D614G; H655Y; N679K; P681H; N764K; D796Y; N856K; Q954H; N969K; L981F 2026-08-15 01:11:25 20
NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF
 
Resource Report
Resource Website
RRID:Addgene_163317 Nsp1, Nsp10, Nsp11 ,Nsp12 Severe acute respiratory syndrome coronavirus 2 Kanamycin Backbone Marker:Promega; Vector Backbone:pFC32K; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin 2026-08-15 01:09:10 0

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