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Plasmids are provided by Addgene and DGRC.

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pMS33
 
Resource Report
Resource Website 		RRID:Addgene_32296 lacZ ORF with short 5'UTR E. coli Ampicillin PMID:22123741 Amplification and sequencing primers for verifying insertion into attTn7: glmS-proximal (Tn7R side) 5’-AATCTGTAACGTTCCGGGTTC-3’ OR 5'-GATGCTGGTGGCGAAGCTGT-3' pstS-proximal (Tn7L side) 5’-CATTAATAACGAAGAGATGAC-3’ OR 5'-GATGACGGTTTGTCACATGGA-3' Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial Expression, bacterial gene addition; Bacterial Resistance:Ampicillin 2026-08-29 01:03:58 0
secretedBirA-8his
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_32408 BirA E. coli Ampicillin PMID:18296487 Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:03:59 7
pVS2
 
Resource Report
Resource Website 		RRID:Addgene_33144 LacO (256 copies) E. coli Ampicillin PMID:18614049 Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable. For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142). pVS2 contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 [Addgene #33143] is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]). pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP. Backbone Marker:Addgene plasmid #33143; Vector Backbone:pVS1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:04:05 0
pCasper_Pdf-LexA::VP16
 
Resource Report
Resource Website 		RRID:Addgene_33259 LexA::VP16-SV40 3'UTR E. coli Ampicillin PMID:19060186 A XbaI site was added to the 3' end of LexAVP16-SV40 3'UTR by PCR from pBS-LexAVP16 plasmid (Lai and Lee, 2006). The PCR fragment was then subcloned into the XbaI site of the pBS vector. LexAVP16-SV40 was then released by NotI digestion and inserted into the NotI site in pCaspeR3, downstream of Pdf promoter. Vector Backbone:pCasPeR3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:04:07 0
pBMN YFP-DHFR(DD)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_29326 YFP-DHFR(DD) E. coli Ampicillin PMID:20851347 Backbone Marker:Garry Nolan; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Val 19 Ala (V19A) and Gly 67 to Ser (G67S)--destabilizing domain 2026-08-29 01:03:34 5
HL 2729 (=HL 716 + rhyB knockout_KanR)
 
Resource Report
Resource Website 		RRID:Addgene_30030 E. coli Kanamycin PMID:21189298 Same stain as HL 2729 except that is not cured of Kanamycin resistence at rhyB Backbone Size:2000; Vector Backbone:Unknown; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-29 01:03:40 0
HL 2818 (=HL 865 + pHL 489)
 
Resource Report
Resource Website 		RRID:Addgene_30032 pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 2305 (=HL 716 + pHL 765)
 
Resource Report
Resource Website 		RRID:Addgene_30020 pLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 2307 (=HL 716 + pHL 777)
 
Resource Report
Resource Website 		RRID:Addgene_30021 pLtetO-1:MicC, pLtetO-1:RhyB, pLlacO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 2312 (=HL 716 + pHL 787)
 
Resource Report
Resource Website 		RRID:Addgene_30024 pLtetO-1:MicC, pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 2314 (=HL 716 + pHL 818)
 
Resource Report
Resource Website 		RRID:Addgene_30025 pLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 2625 (=HL 716 + pHL 908)
 
Resource Report
Resource Website 		RRID:Addgene_30028 pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 2627 (=HL 770 + pHL 908)
 
Resource Report
Resource Website 		RRID:Addgene_30029 pLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 1178 (=HL 862 + pHL 282)
 
Resource Report
Resource Website 		RRID:Addgene_30012 pLtetO-1:mCherry, pLlacO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 1474 (=HL 862 + pHL 404)
 
Resource Report
Resource Website 		RRID:Addgene_30015 pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 2300 (=HL 716 + pHL 745)
 
Resource Report
Resource Website 		RRID:Addgene_30017 pLtetO-1:RhyB-mCherry, pLlacO-1:sodB::gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 3450 (=HL 3262 + pHL 1191)
 
Resource Report
Resource Website 		RRID:Addgene_30080 pLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 3545 (=HL 770 + pHL 1213)
 
Resource Report
Resource Website 		RRID:Addgene_30081 pLlacO-1:hfq, pLtetO-1:RBS (st7) gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0
HL 849 (=HL 716 + micC knockout_KanR)
 
Resource Report
Resource Website 		RRID:Addgene_30009 E. coli Kanamycin PMID:21189298 Same stain as HL 862 except that is not cured of Kanamycin resistence at micC Backbone Size:2000; Vector Backbone:Unknown; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-29 01:03:40 0
HL 3395 (=HL 862 + pHL 1009)
 
Resource Report
Resource Website 		RRID:Addgene_30074 pLlacO-1:MicC-mCherry, pLtetO-1:RBS (st7) gfp E. coli Ampicillin PMID:21189298 Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-29 01:03:40 0

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