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Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599482
Proper citation: RRID:Addgene_22117 Copy
Species: Mus musculus
Genetic Insert: PrP genomic vector with cloning sites
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pBTIIKS(+); Vector Types:construction of targeting vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19709627
Proper citation: RRID:Addgene_22110 Copy
Species: Mus musculus
Genetic Insert: PrP ki-3F4-FFI TC
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBTIIKS(+); Vector Types:Mouse Targeting, construction of targeting vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19709627
Proper citation: RRID:Addgene_22112 Copy
Species: Homo sapiens
Genetic Insert: Ataxin 3
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18599482
Proper citation: RRID:Addgene_22113 Copy
Species: Homo sapiens
Genetic Insert: TETO-eGFP
Vector Backbone Description: Backbone Size:6885; Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19680244
Proper citation: RRID:Addgene_22074 Copy
Species: Homo sapiens
Genetic Insert: SA-Puro
Vector Backbone Description: Backbone Size:5890; Vector Backbone:N/A; Vector Types:ZFN donor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19680244
Comments: Please note that this plasmid contains a non-functional ccdB gene. The use of ccdB survival cells may be needed when subcloning or modifying this vector if the number of transformants is low.
Proper citation: RRID:Addgene_22075 Copy
Vector Backbone Description: Backbone Size:4629; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16294310
Proper citation: RRID:Addgene_22071 Copy
Species: Homo sapiens
Genetic Insert: von Hippel-Lindau tumor suppressor (VHL)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5448; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9651579
Proper citation: RRID:Addgene_22044 Copy
Genetic Insert: mCMV expression cassette
Vector Backbone Description: Backbone Size:6673; Vector Backbone:pAL119; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18287240
Proper citation: RRID:Addgene_22057 Copy
Species: C. reinhardtii
Genetic Insert: ChR2-GFP
Vector Backbone Description: Backbone Marker:Lois/Baltimore; Backbone Size:9115; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16116447
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_22051 Copy
Species: Herpes Simplex Type 1
Genetic Insert: HSV Type 1 thymidine kinase
Vector Backbone Description: Backbone Size:8294; Vector Backbone:pAL120; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18287240
Proper citation: RRID:Addgene_22053 Copy
Species: Herpes Simplex Type 1
Genetic Insert: mCMV-TK-pA expression cassette
Vector Backbone Description: Backbone Size:28750; Vector Backbone:pSTK120; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18287240
Proper citation: RRID:Addgene_22054 Copy
Species: Homo sapiens
Genetic Insert: TFII-I
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10854432
Comments: The internal restriction sites may not be correct
Proper citation: RRID:Addgene_22148 Copy
Species: E. Coli
Genetic Insert: ClpP-His6
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3426; Vector Backbone:pQE70; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16237435
Comments: NGS results indicate S2P in ClpP
Proper citation: RRID:Addgene_22144 Copy
Species: Homo sapiens
Genetic Insert: A20
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18329387
Proper citation: RRID:Addgene_22141 Copy
Species: Homo sapiens
Genetic Insert: partial sequence of human albumine
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pRRL.SIN.cPPT.PGK.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_22037 Copy
Species: Homo sapiens
Genetic Insert: hBEN
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11438732
Comments: GFP cloned in SpeI/ClaI
Proper citation: RRID:Addgene_22158 Copy
Genetic Insert: shlacZ
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pRRL.SIN.cPPT.PGK.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: shLacZ oligo sequence is: 5'-aaaaacgactacacaaatcagcgatttctcttgaaaatcgctgatttgtgtgtcg
Proper citation: RRID:Addgene_22038 Copy
Species: Avena sativa (oat)
Genetic Insert: mVenus-LOV
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBabe-TetCMV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19693014
Comments: Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Proper citation: RRID:Addgene_22033 Copy
Species: Homo sapiens
Genetic Insert: TFII-I
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10854432
Comments: Restriction sites may not be correct.
Proper citation: RRID:Addgene_22154 Copy
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