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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009724394.1
Proper citation: RRID:Addgene_154802 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Spike-delta21
Vector Backbone Description: Backbone Size:4750; Vector Backbone:HDM; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32384820
Comments: Based on a plasmid described previously in Crawford et al. 2020, Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays (https://www.mdpi.com/1999-4915/12/5/513). This is a version of the Spike expression plasmid used to pseudotype lentivirus which provided better titers in this protocol.
Proper citation: RRID:Addgene_155130 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145398 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725303.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145394 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725312.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145392 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp8
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725304.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145393 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724391.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145454 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp10
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725306.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145462 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145480 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S B.1.351
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172736 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172733 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S B.1.427/B.1.429
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172738 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172725 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO/intron; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172723 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 3)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173787 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 4)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173788 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike S-GSAS- P-1-like variant (K417N)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173789 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Backbone Size:6694; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34995482
Proper citation: RRID:Addgene_179905 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Backbone Size:6694; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34995482
Comments: Balazs lab Clone 18
Proper citation: RRID:Addgene_179907 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp1, Nsp10, Nsp11 ,Nsp12
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pFC32K; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_163317 Copy
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