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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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psgRNA Library Resource Report Resource Website |
RRID:Addgene_115927 | E. coli gRNAs | E. coli | Kanamycin | PMID:29765036 | Vector Backbone:psgRNAcos; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin | 2026-08-29 12:52:24 | 0 | ||
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pKSJ340 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27125 | Colicin Ia + Ia immunity protein | E. coli | Ampicillin | PMID:19919671 | The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1) This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C. The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other. | Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:15 | 1 | |
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pBB530 Resource Report Resource Website |
RRID:Addgene_27391 | grpE | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:4493; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:03:18 | 0 | |
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pBB542 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27395 | dnaK, dnaJ, groESL | E. coli | Spectinomycin | PMID:17565681 | Between depositor sequence and Addgene sequencing results, there is a mismatch at position 7284 and an insertion at position 7360. These are in vector backbone and do not alter expression of the inserts. Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:9177; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-29 01:03:18 | 1 | |
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pBB550 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27396 | dnaK, dnaJ, GroESL | E. coli | Spectinomycin | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. single nt insertion at position 1726. | Backbone Size:9203; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin | 2026-08-29 01:03:18 | 3 | |
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pBB540 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27393 | grpE, clpB | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:7194; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:03:18 | 1 | |
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pMA2641 Resource Report Resource Website 1+ mentions |
RRID:Addgene_25435 | reverse tetracycline-regulated transactivator advanced | E. coli | Ampicillin | PMID:19655272 | Backbone Marker:unpublished; Backbone Size:7200; Vector Backbone:pMA2635rc; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-29 01:02:39 | 1 | ||
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pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase Resource Report Resource Website |
RRID:Addgene_25846 | LplA-W37I | E. Coli | Ampicillin | PMID:20534555 | The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression. | Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | W37I | 2026-08-29 01:02:53 | 0 |
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pcDNA3-LplA(W37V) Coumarin PRIME ligase Resource Report Resource Website |
RRID:Addgene_25845 | LplA-W37V | E. Coli | Ampicillin | PMID:20534555 | The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression. | Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | W37V | 2026-08-29 01:02:53 | 0 |
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pMS33 Resource Report Resource Website |
RRID:Addgene_32296 | lacZ ORF with short 5'UTR | E. coli | Ampicillin | PMID:22123741 | Amplification and sequencing primers for verifying insertion into attTn7: glmS-proximal (Tn7R side) 5’-AATCTGTAACGTTCCGGGTTC-3’ OR 5'-GATGCTGGTGGCGAAGCTGT-3' pstS-proximal (Tn7L side) 5’-CATTAATAACGAAGAGATGAC-3’ OR 5'-GATGACGGTTTGTCACATGGA-3' | Backbone Size:13150; Vector Backbone:pMS26; Vector Types:Bacterial Expression, bacterial gene addition; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:58 | 0 | |
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secretedBirA-8his Resource Report Resource Website 1+ mentions |
RRID:Addgene_32408 | BirA | E. coli | Ampicillin | PMID:18296487 | Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:59 | 7 | ||
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pHL1559 Resource Report Resource Website |
RRID:Addgene_37570 | CsrB | E. coli | Kanamycin | PMID:23878244 | Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-29 01:04:42 | 0 | ||
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pHL600 Resource Report Resource Website 1+ mentions |
RRID:Addgene_37563 | CsrB | E. coli | Kanamycin | PMID:23878244 | Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-29 01:04:41 | 1 | ||
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pHL601 Resource Report Resource Website |
RRID:Addgene_37564 | CsrC | E. coli | Kanamycin | PMID:23878244 | Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-29 01:04:42 | 0 | ||
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pHL 279 Resource Report Resource Website |
RRID:Addgene_37757 | ptet_ompC_gfp | E. coli | Ampicillin | PMID:22618873 | Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:42 | 0 | ||
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pHL 363 Resource Report Resource Website |
RRID:Addgene_37758 | pLar_rpoS_gfp | E. coli | Ampicillin | PMID:22618873 | Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:44 | 0 | ||
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pHL 233 Resource Report Resource Website |
RRID:Addgene_37754 | ptet_ompC_gfp | E. coli | Ampicillin | PMID:22618873 | Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin | 2026-08-29 01:04:42 | 0 | ||
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HL 2729 (=HL 716 + rhyB knockout_KanR) Resource Report Resource Website |
RRID:Addgene_30030 | E. coli | Kanamycin | PMID:21189298 | Same stain as HL 2729 except that is not cured of Kanamycin resistence at rhyB | Backbone Size:2000; Vector Backbone:Unknown; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-29 01:03:40 | 0 | ||
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HL 2818 (=HL 865 + pHL 489) Resource Report Resource Website |
RRID:Addgene_30032 | pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp | E. coli | Ampicillin | PMID:21189298 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:40 | 0 | |
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HL 2305 (=HL 716 + pHL 765) Resource Report Resource Website |
RRID:Addgene_30020 | pLtetO-1:MicC, pLtetO-1:DsrA, pLlacO-1:ompC::gfp | E. coli | Ampicillin | PMID:21189298 | Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-29 01:03:40 | 0 |
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