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Species: E. coli
Genetic Insert: GST-YihI with all lysines (K) mutated to arginine (R)
Vector Backbone Description: Backbone Size:4958; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40621900
Comments: Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.
Proper citation: RRID:Addgene_233094 Copy
Species: E. coli
Genetic Insert: YihI
Vector Backbone Description: Backbone Size:4958; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40621900
Comments: Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.
Proper citation: RRID:Addgene_233088 Copy
Species: E. coli
Genetic Insert: NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5409; Vector Backbone:pET21a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40346061
Proper citation: RRID:Addgene_215437 Copy
Species: E. coli
Genetic Insert: PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E)
Vector Backbone Description: Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_238344 Copy
Species: E. coli
Genetic Insert: PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A)
Vector Backbone Description: Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_238343 Copy
Species: E. coli
Genetic Insert: yfp
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 3 parts coding sequences in the CTK system
Proper citation: RRID:Addgene_248444 Copy
Species: E. coli
Genetic Insert: PTet
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2a parts are the first of tandem promoters in the CTK system
Proper citation: RRID:Addgene_248384 Copy
Species: E. coli
Genetic Insert: PSrpR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2a parts are the first of tandem promoters in the CTK system
Proper citation: RRID:Addgene_248386 Copy
Species: E. coli
Genetic Insert: Q1-QacR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Proper citation: RRID:Addgene_248510 Copy
Species: E. coli
Genetic Insert: B3-bm3R1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41581077
Comments: Type 2cd34 parts are expression parts in the CTK system. They just need promoters to be fully functional
Please note: This plasmid contains a T nucleotide deletion in L3S2P55. This deletion is not known to impact plasmid function.
Proper citation: RRID:Addgene_248500 Copy
Species: E. coli
Genetic Insert: E coli csg operon
Vector Backbone Description: Vector Backbone:pTACxc; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40604283
Proper citation: RRID:Addgene_258143 Copy
Species: E. coli
Genetic Insert: Cas3
Vector Backbone Description: Backbone Size:4778; Vector Backbone:pMV_hyg; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37296137
Proper citation: RRID:Addgene_229538 Copy
Species: E. coli
Genetic Insert: McaS
Vector Backbone Description: Backbone Size:3892; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39885279
Comments: Please visit https://doi.org/10.1101/2024.07.23.604302 for bioRxiv preprint.
Proper citation: RRID:Addgene_222403 Copy
Species: E. coli
Genetic Insert: 5’UTR of chiP (-98 to +12, relative to AUG)
Vector Backbone Description: Backbone Size:3933; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:39885279
Comments: Please visit https://doi.org/10.1101/2024.07.23.604302 for bioRxiv preprint.
Proper citation: RRID:Addgene_222404 Copy
Species: E. coli
Genetic Insert: EcTyrRS
Vector Backbone Description: Vector Backbone:pAcBac1; Vector Types:Mammalian Expression, Baculoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36905325
Proper citation: RRID:Addgene_228782 Copy
Species: E. coli
Genetic Insert: GST
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5366; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37638647
Proper citation: RRID:Addgene_225224 Copy
Species: E. coli
Genetic Insert: FkpA peptidyl-prolyl cis-trans isomerase FkpA
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16720693
Proper citation: RRID:Addgene_230905 Copy
Species: E. coli
Genetic Insert: holD (psi)
Vector Backbone Description: Vector Backbone:pET-3c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_237236 Copy
Species: E. coli
Genetic Insert: holB (delta prime)
Vector Backbone Description: Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Aat II, Cla I, & Hind III sites eliminated from plasmid backbone
Proper citation: RRID:Addgene_237234 Copy
Species: E. coli
Genetic Insert: dnaN (beta), N-terminal his-kinase cleavable tag
Vector Backbone Description: Vector Backbone:pET-16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The PKA kinase site and PreScission Protesase clevage site are added between the his-10 tag & Factor Xa sequence in pET(16b) and the start of the Ec dnaN ORF.
Proper citation: RRID:Addgene_237239 Copy
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