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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
IRE1b(K536A).9E10.pCDNA3
 
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RRID:Addgene_21881 IRE1b Mus musculus Ampicillin Addgene sequencing results show an additional mutation in the coding region, causing L850P substitution in the amino acid sequence. Mouse IRE1b, K536A, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3. The K->A mutation introduces a second MSc1 site into the plasmid at position 2578. Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K536A 2026-07-25 12:44:28 0
boP58-pBabe.puro(notag)
 
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RRID:Addgene_21885 P58 Ampicillin PMID:16923392 Addgene sequencing results show there is no HA tag in this plasmid. The start codon of the gag gene has been mutated so that it does not get expressed. Its presence, however,increases the level of retroviral production. Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 0
U6+27-20pf
 
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RRID:Addgene_21978 U6 miR-20 perfect Ampicillin PMID:17694064 miR-20 perfect CMV sponge and U6 sponge, 2 sites: CUACCUGCACUAUAAGCACUUUA Backbone Size:5000; Vector Backbone:U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:29 0
U6+27-20
 
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RRID:Addgene_21977 U6 miR-20 sponge Ampicillin PMID:17694064 Backbone Size:5000; Vector Backbone:U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:29 0
U6+27-16pf
 
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RRID:Addgene_21976 U6 miR-16 perfect Ampicillin PMID:17694064 miR-16 perfect CMV sponge and U6 sponge: CGCCAUAUUUACGUGCUGCUA Backbone Size:5000; Vector Backbone:U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:29 0
U6+27-16
 
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RRID:Addgene_21975 U6 miR-16 sponge Ampicillin PMID:17694064 mir-16 bulged Renilla luciferase reporter, CMV sponge, and U6 sponge, 9 sites AAUAUUCUAUGCUGCUA Backbone Size:5000; Vector Backbone:U6+27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:29 0
pTriEx-mCherry-PA-Rac1
 
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10+ mentions
RRID:Addgene_22027 PA-Rac1 Homo sapiens Ampicillin PMID:19693014 Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments! Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt. Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin LOV(Leu404-Leu546), Rac1 starts at Ile4 and contains mutations Q61L, E91H and N92H 2026-07-25 12:44:29 22
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
 
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RRID:Addgene_22023 mVenus-PA-Rac1-C450A Homo sapiens Ampicillin PMID:19693014 Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments! Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt. Backbone Size:6000; Vector Backbone:pBabe-TetCMV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin The LOV domain contains the mutation C450A.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 2026-07-25 12:44:29 0
pTriEx-mVenus-PA-Rac1-C450A
 
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1+ mentions
RRID:Addgene_22021 PA-Rac1-C450A Homo sapiens Ampicillin PMID:19693014 Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments! Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt. Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin LOV domain contains the light-insensitive C450A mutation. Rac1 starts at I4 and contains mutations Q61L, E91H and N92H. 2026-07-25 12:44:29 2
pAL119
 
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RRID:Addgene_21909 hCMV promoter expression cassette Ampicillin PMID:15564139 Backbone Size:6700; Vector Backbone:pAL; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 0
pSicoR-mCh-Oct4i
 
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1+ mentions
RRID:Addgene_21906 Oct4i Mus musculus Ampicillin PMID:19587682 Oct4 RNAi sequence 5'-GAACCTGGCTAAGCTTCCA Backbone Size:7558; Vector Backbone:pSicoR; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 1
9E10.mCHOP(S78&81A).pCDNA1/Amp
 
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1+ mentions
RRID:Addgene_21902 CHOP Mus musculus Ampicillin 9E10 (Myc-epitope) tagged mouse CHOP coding region only, S78A and S81A double mutant. The S78A and S81A mutation introduces an additional HhaI site not present in the wildtype insert. Note that there is considerable uncertainity regarding this plasmid. Backbone Size:4767; Vector Backbone:pcDNA1.1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S78A S81A 2026-07-25 12:44:28 1
T7-RelA
 
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1+ mentions
RRID:Addgene_21984 NFkB p65 subunit Homo sapiens Ampicillin PMID:11533489 Backbone Marker:Matthias P et al. Nucleic Acids Research (1989). 17(15): 6418; Backbone Size:5120; Vector Backbone:pEv3s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin For more details for this expression construct, as well as associated mutants, please contact: Dr. Lin-Feng Chen ([email protected]) 2026-07-25 12:44:29 8
pFLAG-6a-Ataxin3Q22-UIM*
 
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RRID:Addgene_22128 Ataxin 3 Homo sapiens Ampicillin Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV-6a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin UIM 1,2,3 SA-DG 2026-07-25 12:44:30 0
pVETL-Cmcs-Flag-Ataxin3Q80-C14A
 
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RRID:Addgene_22121 Ataxin 3 Homo sapiens Ampicillin PMID:17693639 Backbone Size:5756; Vector Backbone:pVETL-Cmcs; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin C14A 2026-07-25 12:44:30 0
cFGF8
 
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RRID:Addgene_22089 FGF8 Gallus gallus Ampicillin PMID:8548816 Chick homolog of mouse FGF8 splice variant 1 clone was isolated from an E10 chick brain cDNA library directionally cloned into lambdaZapII. The insert contains most of the coding region. For antisense probe linearize with EcoRI and use T7 Polymerase. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK (-); Vector Types:Bacterial; Bacterial Resistance:Ampicillin 2026-07-25 12:44:30 0
mFGF2
 
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RRID:Addgene_22084 FGF2 Mus musculus Ampicillin Full length coding region, PCR clone. The insert can be cut out with EcoRI and XbaI. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial; Bacterial Resistance:Ampicillin 2026-07-25 12:44:30 0
mFGF3
 
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RRID:Addgene_22086 FGF3 Mus musculus Ampicillin cDNA with longest 5' end; poly A tail lost because cut with EcoRI. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial; Bacterial Resistance:Ampicillin 2026-07-25 12:44:30 0
pBS119d10L
 
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RRID:Addgene_22081 pBS119d10L Danio rerio Ampicillin PMID:12167406 To view partial vector sequence from the depositing lab, please click on the Reviews link. Backbone Marker:Strategene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:30 0
HA-HIF2α MT-pBabe-Hygro
 
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RRID:Addgene_22134 Hypoxia inducible factor 2 alpha Homo sapiens Ampicillin PMID:14691554 Backbone Size:5573; Vector Backbone:pBabe-Hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin cDNA "silent"("conservative") mutations: GGAGACGGAGGTGTTCTAT mutated to GGAGACCGAAGTCTTCTAT to render cDNA resistant to siRNA #3: 5’-GGAGACGGAGGTGTTCTAT-3’ 2026-07-25 12:44:30 0

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