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Species:
Genetic Insert: p53-binding site (mutant)
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream.
MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies.
Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG-
3') were used for the
p53 nonbinding control sequences.
Proper citation: RRID:Addgene_16443 Copy
Species: Homo sapiens
Genetic Insert: MDM2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length human MDM2 cDNA.
Proper citation: RRID:Addgene_16441 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The vector pCMV-Neo-Bam was engineered to contain two independent transcription units. The first unit is comprised of a CMV promoter/enhancer upstream of a multiple cloning site, and splice and polyA sites to ensure appropriate processing. The second transcription unit includes a herpes simplex virus (HSV) thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
Proper citation: RRID:Addgene_16440 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:11536; Vector Backbone:lentiCRISPRV2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164273 Copy
Species:
Genetic Insert: SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164437 Copy
Species: Homo sapiens
Genetic Insert: WAF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10400; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: full-length WAF1 cDNA insert cloned into the NotI site of pCEP4.
Proper citation: RRID:Addgene_16450 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3726; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
References:
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD123.102, Ligation number L4138.
Proper citation: RRID:Addgene_1645 Copy
Species: Mus musculus
Genetic Insert: Tgfb1
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164427 Copy
Species: Mus musculus
Genetic Insert: CD200
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164425 Copy
Species: Mus musculus
Genetic Insert: Tgfb3
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6062; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164429 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_451m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164452 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_453m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164453 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-mscRE4-minCMV-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Funded by BRAIN initiative.
The Allen Institute for Brain Science recommends this plasmid as a potential substitute for Plasmid 163471: AiP1269-scAAV-mscRE4-minBGpromoter-SYFP2-WPRE3-bGHpA.
Proper citation: RRID:Addgene_164457 Copy
Species: Synthetic
Genetic Insert: SYFP2
Vector Backbone Description: Backbone Marker:Allen Institute for Brain Science; Vector Backbone:rAAV-eHGT_460m-minBglobin-SYFP2-WPRE3-BGHpA; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments: Funded by BRAIN initiative.
Proper citation: RRID:Addgene_164455 Copy
Species:
Genetic Insert: VSV-G
Vector Backbone Description: Vector Backbone:HDM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164440 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164445 Copy
Species: Homo sapiens
Genetic Insert: p53
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: A 1.8kb XbaI fragment encoding wild-type p53 (from nucleotide -130 to 1671 relative to the ATG) was blunt-ended and ligated to BamHI linkers. This was then cloned into the unique BamHI site in the pCMV-Neo-Bam vector. This plasmid is also known as pC53-SN3.
Proper citation: RRID:Addgene_16434 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-ALIX(703-868, P801G)-Strep
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164444 Copy
Species: Caenorhabditis elegans
Genetic Insert: Tandem pore domain K+ channel twk-18
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pOX; Vector Types:Xenopus Oocyte expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16430 Copy
Species: Homo sapiens
Genetic Insert: GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep
Vector Backbone Description: Backbone Size:5643; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164447 Copy
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