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Species: Homo sapiens
Genetic Insert: JARID2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114443 Copy
Species: Homo sapiens
Genetic Insert: PALI1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114442 Copy
Species: Homo sapiens
Genetic Insert: LCOR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114441 Copy
Species: Mus musculus
Genetic Insert: Microtubule binding domain
Vector Backbone Description: Vector Backbone:pBART27 R4R3; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29739839
Comments: Addgene QC results discovered an IS1 inserted into the plasmid backbone. Please review the sequence for further information.
Proper citation: RRID:Addgene_115222 Copy
Species: Homo sapiens
Genetic Insert: PARK7C53A
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31536960
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115179 Copy
Species: Homo sapiens
Genetic Insert: PARK7E163K
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31536960
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115181 Copy
Genetic Insert: g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
Vector Backbone Description: Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30760722
Proper citation: RRID:Addgene_115484 Copy
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10759496
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article.
Kanamycin gene for plant selection on MS media.
Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l).
The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.
Proper citation: RRID:Addgene_11572 Copy
Species: Arabidopsis thaliana
Genetic Insert: pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_115995 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116013 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116016 Copy
Species: Arabidopsis thaliana
Genetic Insert: pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116010 Copy
Species: Arabidopsis thaliana
Genetic Insert: pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116012 Copy
Species: Arabidopsis thaliana
Genetic Insert: pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116006 Copy
Species: Arabidopsis thaliana
Genetic Insert: pSMXL5:GR-LhG4:tSMXL5:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116007 Copy
Species: Arabidopsis thaliana
Genetic Insert: pNST3:GR-LhG4:tNST3:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z003; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_116009 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
Vector Backbone Description: Vector Backbone:Z001; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30026289
Proper citation: RRID:Addgene_115998 Copy
Species: Other
Genetic Insert: AtDeadCAS9 D10A H840A without stop codon
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112073 Copy
Species: Other
Genetic Insert: 3xmVenus
Vector Backbone Description: Vector Backbone:pAGM1301; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112074 Copy
Species: Other
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pICH41258; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis.
Proper citation: RRID:Addgene_112080 Copy
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