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Species: Homo sapiens
Genetic Insert: PA-Rac1-T17N
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt.
Proper citation: RRID:Addgene_22017 Copy
Species: Homo sapiens
Genetic Insert: mVenus-PA-Rac1-T17N
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBabe-TetCMV-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt.
Proper citation: RRID:Addgene_22018 Copy
Species:
Genetic Insert: Ires GFP
Vector Backbone Description: Backbone Size:11476; Vector Backbone:LZRS-pBMN-Z-delta; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21961 Copy
Species: Mus musculus
Genetic Insert: Pten
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21960 Copy
Species: Homo sapiens
Genetic Insert: BRD4
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21939 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
Proper citation: RRID:Addgene_21899 Copy
Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4310; Vector Backbone:pGL3 and pGL2 modified; Vector Types:Non-viral; Bacterial Resistance:Ampicillin
References:
Comments: This reporter gene closely tracks the expression of endogenous CHOP.
Proper citation: RRID:Addgene_21898 Copy
Species: Caenorhabditis elegans
Genetic Insert: BiP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: This reporter gene tracks the expression of endogenous BiP (hsp-4) in C. elegans. It consists of a ~1.1 KB genomic C. elegans fragment linked to GFP in the vector pPD95.75. The fusion between the GFP artificial gene and hsp-4 takes place at AA #3 of HSP-4)
Proper citation: RRID:Addgene_21896 Copy
Species:
Genetic Insert: EF1 alpha -miR26a GFP p(A)
Vector Backbone Description: Backbone Size:3952; Vector Backbone:pEMBL; Vector Types:Mammalian Expression, AAV, sc; Bacterial Resistance:Ampicillin
References:
Comments: miR-26a sequence is 5'-TTCAAGTAATCCAGGATAGGC
Addgene sequencing results confirm the presence of both FseI sites flanking miR-26a.
Proper citation: RRID:Addgene_21894 Copy
Species: Homo sapiens
Genetic Insert: BRD4
Vector Backbone Description: Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21943 Copy
Species: Homo sapiens
Genetic Insert: BRD4
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21942 Copy
Species: Homo sapiens
Genetic Insert: betaActin
Vector Backbone Description: Backbone Size:5802; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21949 Copy
Species: jellyfish
Genetic Insert: mGFP-10-sREACh
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:3935; Vector Backbone:mGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21947 Copy
Species: Homo sapiens
Genetic Insert: Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21919 Copy
Species: Homo sapiens
Genetic Insert: Full length human HKI in pGFP-N3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21917 Copy
Species: Homo sapiens
Genetic Insert: Human Soluble Fms-like Tyrosine Kinase 3 Ligand
Vector Backbone Description: Backbone Size:7300; Vector Backbone:pAL119; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21910 Copy
Species: Mus musculus
Genetic Insert: CHOP
Vector Backbone Description: Backbone Size:4767; Vector Backbone:pcDNA1.1/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing results show 6bp insertion in the sequencing adding 2 Gly residues immediately after the Myc tag.
Mammalian expression plasmid encoding wildtype mouse CHOP fused to DNA-binding domain of yeast Gal4.
Proper citation: RRID:Addgene_21914 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments: From depositing lab:
"9E10 (Myc-epitope) tagged mouse CHOP coding region only. The insert was joined to the epitope tag using patch-PCR and incorporating unique BamH1 and Xho1 sites for ligation into pcDNA1. Stocks are provided as MC1060 (or MC1061, we are not sure) host strain transformed with the plasmid. Note that pCDNA1 plasmids must be propagated on combined amp and tet selection. I would recommedn that you consider transferring the insert into a better host strain such as pcDNA3"
Proper citation: RRID:Addgene_21913 Copy
Species: Homo sapiens
Genetic Insert: Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21922 Copy
Species: Homo sapiens
Genetic Insert: hnRNPF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4700; Vector Backbone:p3xFLAG-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21926 Copy
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