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Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178155 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178152 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178153 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12778056
Comments: does not contain lacI/
ERG1, ERG8, MVD1: S. cerevisiae/
idi: E. Coli
Proper citation: RRID:Addgene_17816 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MevT
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBAD24; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17239639
Proper citation: RRID:Addgene_17820 Copy
Species: E. coli
Genetic Insert: dxs
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:0; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11180061
Proper citation: RRID:Addgene_17821 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178143 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178144 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178218 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178216 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178170 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178175 Copy
Species: Homo sapiens
Genetic Insert: nPC Tagged mCherry-NLS
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLKO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35290801
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.13.451021v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178209 Copy
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35074588
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.10.18.464913v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178203 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178168 Copy
Species: C.intestinalis, E. quadricolor
Genetic Insert: rArc
Vector Backbone Description: Vector Backbone:pCDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34620975
Proper citation: RRID:Addgene_178206 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178161 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178165 Copy
Species: Homo sapiens
Genetic Insert: Halotag
Vector Backbone Description: Vector Backbone:pUC57-BsaI-Free; Vector Types:Donor vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178163 Copy
Species: Homo sapiens
Genetic Insert: Autotaxin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:pSecTag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327261
Comments: From the depositor:
In 1998, we purchased from Invitrogen pSecTag2A and cut it with BglII and BamH1. The BglII/BamH1 fragment was replaced with a modified fragment containing 6 Histidines at the N-Terminus that was done by generating the modification by PCR, and cloning the PCR product back into BglII/BamH1.
Sometime later, the above pSecTag2A-6HIS was digested with BamH1and Not1. The ATX (amino acids 52-863) insert was genereated by PCR, digested with BamH1/EcoR1 and by excising ATX from the original colony II cDNA library with EcoR1/Not1, which yields two pieces in two stages into the vector.
Their is a stop codon before the C-Terminus Myc tag so it will not be expressed.
Proper citation: RRID:Addgene_17839 Copy
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