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http://www.addgene.org/21828

Species: Homo sapiens
Genetic Insert: TLS
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Human TLS full-length cDNA in pCDNA1 expression vector.

Proper citation: RRID:Addgene_21828 Copy   

•   Source: Addgene

http://www.addgene.org/21826

Species: simian virus
Genetic Insert: SV40 Large T
Vector Backbone Description: Backbone Size:3200; Vector Backbone:Bluescribe; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: SV40 large T antigen for immortalizing cells. See Author's Map for more information. Please note that this plasmid likely expreses a truncated SV40 large T antigen protein starting with the methionine at amino acid 109, when compared to GenBank reference sequence YP_003708382.1 (see NC_001669.1 for the corresponding nucleotide sequence). The plasmid functions as described to immortalize MEFs.

Proper citation: RRID:Addgene_21826 Copy   

•   Source: Addgene

http://www.addgene.org/21758

Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21758 Copy   

•   Source: Addgene

http://www.addgene.org/21756

Species: Homo sapiens
Genetic Insert: Ataxin-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21756 Copy   

•   Source: Addgene

http://www.addgene.org/21871

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3508; Vector Backbone:pMLM36; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Combinatorial libraries of zinc finger arrays made by OPEN are cloned into this plasmid (see Maeder et al., Mol Cel 2008, PMID 18657511). Ligate zinc finger array fragments with BbsI-digested pBR-UV5-GP-FD2 vector backbone to create plasmids that express the zinc finger array as a FLAG-tagged Gal11P fusion in the B2H system. The lacUV5 promoter which drives expression of the Gal11P fusion protein contains a lac operator and therefore can be repressed by lac repressor. This plasmid is a low copy number plasmid and therefore we recommend that minipreps be performed from a 4 to 10 ml culture. Annotations for pMLM36: 12-17: lacUV5 promoter -35 box / 36-41: lacUV5 promoter -10 box / 48: lacUV5 promoter transcription start point / 86-88: Start codon for Gal11P protein fragment / 89-358: Coding sequence for Gal11P a.a. 263-352 (with additional translationally silent mutations) / 407-412: BbsI site #1 / 433-438: BbsI site #2 / 568-1024: f1 phage origin of replication / 1156-2013: beta-lactamase gene cassette (confers resistance to ampicillin and carbenicillin) / 2016-2956: ColE1 origin of replication (plasmid origin) / 3203-3390: rop gene (associated with regulation of copy number)

Proper citation: RRID:Addgene_21871 Copy   

•   Source: Addgene

http://www.addgene.org/21875

Species:
Genetic Insert: CSH100 bacterial Strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
References:
Comments: F’ lac proA+proB+(lacIq lacPL8)/ara- ∆(gpt-lac)5 This strain will be shipped as bacteria in an LB stab. Upon receipt, requesting scientists should restreak the strain on an M9 minimal plate. After restreaking on M9 to confirm the presence of the F', scientists can grow the strain in liquid LB. M9 minimal medium agar plates: To prepare 500 ml, autoclave 439 ml H2O with 7.5 g Bacto-agar and a stir bar. When agar has cooled to approximately 65°C, add 50 ml 10X M9 salts, 1 ml 1 M MgSO4, 10 ml 20% (w/v) glucose and 0.5 ml 100mM CaCl2 and then pour plates. Plates can be stored indefinitely at 4°C in sealed plastic bags. (Alternatively, M9 plates can be purchased from Teknonva: https://www.teknova.com/content/teknova/us/en/products/product-page.html/m1260.html).

Proper citation: RRID:Addgene_21875 Copy   

•   Source: Addgene

http://www.addgene.org/21874

Species:
Genetic Insert: KJ1C bacterial Strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Tetracycline
References:
Comments: F- ∆hisB463 ∆(gpt-proAB-arg-lac)XIII zaj::Tn10

Proper citation: RRID:Addgene_21874 Copy   

•   Source: Addgene

http://www.addgene.org/21873

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5725; Vector Backbone:pMLM292; Vector Types:Mammalian Expression, zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments: Zinc fingers are cloned in between the unique XbaI and BamHI sites. This results in an expression vector which encodes a ZFN with the OPEN zinc finger array fused to the mutant FokI nuclease domain. pMLM292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains 235- 822: CMV promoter / 771-775: CAAT box / 804-808: TATA box / 819: 3' end of hCMV / 904-906: ZFN start codon / 910-930: SV40 nuclease localization signal (NLS) / 940- 963: FLAG tag / 964- 969: XbaI site / 980- 985: BamHI site / 986-1573: FokI nuclease domain / 1292&1294: Q486E heterodimer FokI mutation / 1331: I499L heterodimer FokI mutation / 1574-1576: ZFN stop codon / 1686-1910: BGH polyadenylation sequence / 1956-2384: f1 origin / 2411-2720: SV40 early promoter and origin / 2775-2841: EM-7 Promoter / 2842-3240: Blasticidin resistance gene / 3398-3528: SV40 late polyA signal / 3955-4574: pUC origin / 4729-5589: ampicillin (bla) resistance gene (complementary strand) / 5590-5688: bla promoter (complementary strand)

Proper citation: RRID:Addgene_21873 Copy   

•   Source: Addgene

http://www.addgene.org/21802

Species: Homo sapiens
Genetic Insert: SHP-1
Vector Backbone Description: Backbone Size:7767; Vector Backbone:pcDNA-Dest53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21802 Copy   

•   Source: Addgene

http://www.addgene.org/21801

Species: Mus musculus
Genetic Insert: PSMD14
Vector Backbone Description: Backbone Marker:Clontech (?); Backbone Size:4200; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that though there is a Kan resistance gene in this plasmid according to the author's sequence, the gene is not functional. This plasmid does NOT confer Kan resistance.

Proper citation: RRID:Addgene_21801 Copy   

•   Source: Addgene

http://www.addgene.org/21800

Species: Mus musculus
Genetic Insert: PSMD14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21800 Copy   

•   Source: Addgene

http://www.addgene.org/21766

Species:
Genetic Insert: ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_21766 Copy   

•   Source: Addgene

http://www.addgene.org/21806

Species: Homo sapiens
Genetic Insert: SHP-1
Vector Backbone Description: Backbone Size:7420; Vector Backbone:pDest472; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21806 Copy   

•   Source: Addgene

http://www.addgene.org/21804

Species: Homo sapiens
Genetic Insert: SHP-1
Vector Backbone Description: Backbone Size:7420; Vector Backbone:pDest472; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21804 Copy   

•   Source: Addgene

http://www.addgene.org/21761

Species:
Genetic Insert: ECFP -EYFP linked by flexible linker containing two SGGSGG repeats
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_21761 Copy   

•   Source: Addgene

http://www.addgene.org/21760

Species:
Genetic Insert: ECFP -EYFP linked by flexible one SGGSGG repeats
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_21760 Copy   

•   Source: Addgene

http://www.addgene.org/21880

Species: Mus musculus
Genetic Insert: IRE1b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse IRE1b, wild-type, 9E10-tagged at C-term, assembled from lambda-phage clones 37 and 26, in pCDNA3.

Proper citation: RRID:Addgene_21880 Copy   

•   Source: Addgene

http://www.addgene.org/21763

Species:
Genetic Insert: ECFP -EYFP linked by flexible linker containing 4 SGGSGG repeats
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_21763 Copy   

•   Source: Addgene

http://www.addgene.org/21762

Species:
Genetic Insert: ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_21762 Copy   

•   Source: Addgene

http://www.addgene.org/21857

Species: Mus musculus
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: These pEGFP-based plasmids have NEOr marker (and not the conventional AMPr marker). They should be propagated in the presence of 30mcg/ml kanamycin (and not on ampicillin)

Proper citation: RRID:Addgene_21857 Copy   

•   Source: Addgene