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Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:4636; Vector Backbone:pCMV-7.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23942116
Comments: This plasmid can be used to isolate specific genomic regions of interest using a catalytically inactive Cas9 fused with a tag(s).
Purify: locus-specific chromatin immunoprecipitation (enChIP)
This system is compatible with gRNA_cloning_vector (www.addgene.org/41824) from the Church lab.
Additional information and protocols can be found at:
http://www.med.hirosaki-u.ac.jp/~bgb/iChIP_protocols/index_e.html
Proper citation: RRID:Addgene_47948 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23979586
Comments: To generate the unique SpeI site, a short, 37 bp, linker was inserted into an existing NdeI site. This linker seems to have been inserted 2 or more times, creating a short repeat region that we have been unable to sequence across. Sequencing with the M13 reverse primer will fail.
Proper citation: RRID:Addgene_47946 Copy
Species: Rattus norvegicus
Genetic Insert: SAPAP1
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115257
Proper citation: RRID:Addgene_47940 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23979586
Comments: To generate the unique SpeI site, a short, 37 bp, linker was inserted into an existing NdeI site. This linker seems to have been inserted 2 or more times, creating a short repeat region that we have been unable to sequence across. Sequencing with the M13 reverse primer will fail.
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_47944 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK+; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23979586
Comments: To generate the unique SpeI site, a short, 37 bp, linker was inserted into an existing NdeI site. This linker seems to have been inserted 2 or more times, creating a short repeat region that we have been unable to sequence across. Sequencing with the M13 reverse primer will fail.
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_47945 Copy
Species: Synthetic
Genetic Insert: guide RNA
Vector Backbone Description: Backbone Marker:Geneart; Vector Backbone:pMK; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23979586
Proper citation: RRID:Addgene_47943 Copy
Species: Homo sapiens
Genetic Insert: MOB1A
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19919647
Proper citation: RRID:Addgene_47937 Copy
Species: Homo sapiens
Genetic Insert: CIN85
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751601
Comments: The CIN85 insert in this plasmid contains a D114G mutation when compared to GenBank accession number NP_114098.1. This mutation was not intentional and was present in the original CIN85 clone.
Proper citation: RRID:Addgene_47935 Copy
Species: Homo sapiens
Genetic Insert: MOB1A
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19919647
Proper citation: RRID:Addgene_47936 Copy
Species: Homo sapiens
Genetic Insert: Miro2 A13V
Vector Backbone Description: Backbone Marker:Dr. Alan Hall, MRC-LMCB; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630562
Proper citation: RRID:Addgene_47896 Copy
Species: Homo sapiens
Genetic Insert: Miro2 T18N
Vector Backbone Description: Backbone Marker:Dr. Alan Hall, MRC-LMCB; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630562
Proper citation: RRID:Addgene_47897 Copy
Species: Danio rerio
Genetic Insert: golden gRNA
Vector Backbone Description: Backbone Size:2542; Vector Backbone:pT7-gRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23918387
Comments: For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/
gRNA target sequence GGTCTCTCGCAGGATGTTGC
Proper citation: RRID:Addgene_47930 Copy
Species: Homo sapiens
Genetic Insert: Miro1 E208K/E328K
Vector Backbone Description: Backbone Marker:Dr. Alan Hall, MRC-LMCB; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16630562
Proper citation: RRID:Addgene_47894 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2730; Vector Backbone:pUC57; Vector Types:CRISPR, Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23979578
Proper citation: RRID:Addgene_47933 Copy
Species: Homo sapiens
Genetic Insert: Dendrin-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16751601
Proper citation: RRID:Addgene_47934 Copy
Species: Danio rerio
Genetic Insert: mitfa gRNA
Vector Backbone Description: Backbone Size:2542; Vector Backbone:pT7-gRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23918387
Comments: For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/
gRNA target sequence GGTCTCTCGCAGGATGTTGC
Proper citation: RRID:Addgene_47931 Copy
Species: Danio rerio
Genetic Insert: ddx19 gRNA
Vector Backbone Description: Backbone Size:2542; Vector Backbone:pT7-gRNA; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23918387
Comments: For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/
gRNA target sequence GGCAACAGATTCGTGGGCCC
Proper citation: RRID:Addgene_47932 Copy
Species: Homo sapiens
Genetic Insert: Miro2
Vector Backbone Description: Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12482879
Proper citation: RRID:Addgene_47891 Copy
Species: Mus musculus
Genetic Insert: E-Cadherin EC12
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5406; Vector Backbone:pET-22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23923816
Comments: Plasmid contains E-Cadherin domains 1 and 2 (aa157-375, when compared to GenBank reference sequence NP_033994.1). Numbering of the mutations is relative to this sequence. The pro-peptide sequence that is normally cleaved during maturation was intentionally removed as part of the cloning strategy for expression in bacteria.
Proper citation: RRID:Addgene_47926 Copy
Species: Mus musculus
Genetic Insert: E-Cadherin EC12
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5406; Vector Backbone:pET-22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23923816
Comments: Plasmid contains E-Cadherin domains 1 and 2 (aa157-375, when compared to GenBank reference sequence NP_033994.1). Numbering of the mutation is relative to this sequence. The pro-peptide sequence that is normally cleaved during maturation was intentionally removed as part of the cloning strategy for expression in bacteria.
Proper citation: RRID:Addgene_47924 Copy
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