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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-D614G variant)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33417835
Proper citation: RRID:Addgene_164566 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike - Furin site changed for HRV3C site
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33417835
Proper citation: RRID:Addgene_164567 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-RRAR variant)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33417835
Proper citation: RRID:Addgene_164569 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 3)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173787 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-B.1.351 variant version 4)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173788 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike S-GSAS- P-1-like variant (K417N)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168071
Proper citation: RRID:Addgene_173789 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike RBD N343Q, E484K
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34416153
Proper citation: RRID:Addgene_174298 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: CoV2-RBD-beta variant
Vector Backbone Description: Vector Backbone:pcDNA216; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34723224
Proper citation: RRID:Addgene_194598 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Backbone Size:6694; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34995482
Proper citation: RRID:Addgene_179905 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids
Vector Backbone Description: Backbone Marker:Twist; Backbone Size:6694; Vector Backbone:pTwist-CMV-BetaGlobin-WPRE-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34995482
Comments: Balazs lab Clone 18
Proper citation: RRID:Addgene_179907 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-OMICRON)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35447081
Comments: See this as a reference: https://www.addgene.org/164566/.
Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477784v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_180423 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-RRAR-OMICRON)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35447081
Comments: See this as a reference: https://www.addgene.org/164566/.
Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477784v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_180424 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp15-H234A
Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40411374
Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-H234A (amino acid residues 6453-6798 of YP_009724389, with H234A mutation).
Confers ampicillin-resistance.
Backbone vector is pET-11b from GenScript.
Mutation design by Nirupa Nagaratnam.
Proper citation: RRID:Addgene_219529 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp13_SARS2
Vector Backbone Description: Vector Backbone:pFBD-BirA; Vector Types:Baculovirus Expresssion; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234375 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp7_SARS2
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_234405 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp3_SARS2
Vector Backbone Description: Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_234389 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Nsp14_SARS2
Vector Backbone Description: Vector Backbone:p28BIOH-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_234376 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 Mpro
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34437808
Comments: SUMO fusion can be removed by cleavage with Ulp1 protease
Proper citation: RRID:Addgene_172653 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA.1
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35732171
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184831 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Spike (S-GSAS-rS2d.HexaPro)
Vector Backbone Description: Backbone Size:4746; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35447081
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.25.477784v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_183515 Copy
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