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Species: Synthetic
Genetic Insert: Fusicatenibacter saccharivorans RT-Cas1-Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30283135
Comments: Please see the annotated GenBank file and the additional image file for the location of specific features in the sequence of this plasmid.
Proper citation: RRID:Addgene_116965 Copy
Species: Synthetic
Genetic Insert: gDmap1 v4
Vector Backbone Description: Backbone Size:8629; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene: 67974); Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30932271
Proper citation: RRID:Addgene_117143 Copy
Species: Synthetic
Genetic Insert: gDmap1 v5
Vector Backbone Description: Backbone Size:8629; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W (Addgene: 67974); Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30932271
Proper citation: RRID:Addgene_117144 Copy
Species: Synthetic
Genetic Insert: BSD2A
Vector Backbone Description: Backbone Size:9123; Vector Backbone:pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_117136 Copy
Species: Synthetic
Genetic Insert: gCD44 v2
Vector Backbone Description: Backbone Size:14044; Vector Backbone:pKLV2-U6(BsmBI)-EF1a-BFP-Puro-Cas9(FZ); Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_117134 Copy
Species: Synthetic
Genetic Insert: gRNA miR-124-1-3'
Vector Backbone Description: Backbone Marker:Zhang Lab; Backbone Size:9157; Vector Backbone:PX459; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30292704
Comments: Cas9 with NLS included
Proper citation: RRID:Addgene_117324 Copy
Species: Synthetic
Genetic Insert: SauCas9 sgRNA targeting TLR 2.0
Vector Backbone Description: Backbone Marker:Addgene#50920; Vector Backbone:pLKO.1-puro-U6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36070530
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117409 Copy
Species: Synthetic
Genetic Insert: SpyCas9 sgRNA targeting TLR 2.0
Vector Backbone Description: Backbone Marker:Addgene#50920; Vector Backbone:pLKO.1-puro-U6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36070530
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117406 Copy
Species: Synthetic
Genetic Insert: mPlum
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30301859
Proper citation: RRID:Addgene_117396 Copy
Species: Synthetic
Genetic Insert: dTomato
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30301859
Proper citation: RRID:Addgene_117395 Copy
Species: Synthetic
Genetic Insert: mPlum
Vector Backbone Description: Vector Backbone:pGEN-mcs (ori15A); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30301859
Proper citation: RRID:Addgene_117388 Copy
Species: Synthetic
Genetic Insert: GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30626963
Proper citation: RRID:Addgene_117384 Copy
Species: Synthetic
Genetic Insert: mPlum
Vector Backbone Description: Vector Backbone:pUC18R6K; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30301859
Proper citation: RRID:Addgene_117392 Copy
Species: Synthetic
Genetic Insert: dTomato
Vector Backbone Description: Vector Backbone:pUC18R6K; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30301859
Proper citation: RRID:Addgene_117391 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30301859
Proper citation: RRID:Addgene_117394 Copy
Species: Synthetic
Genetic Insert: AsCas12a gRNA targeting TLR 2.0
Vector Backbone Description: Backbone Marker:Addgene#50920; Vector Backbone:pLKO.1-puro-U6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36070530
Comments: Plasmid contains an IS1 prokaryotic transposable element that does not affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117411 Copy
Species: Synthetic
Genetic Insert: FnCas12a gRNA targeting TLR 2.0
Vector Backbone Description: Vector Backbone:pBluescript SK (+); Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36070530
Comments: Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117412 Copy
Species: Synthetic
Genetic Insert: EYFP
Vector Backbone Description: Backbone Size:4751; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30626963
Proper citation: RRID:Addgene_117383 Copy
Species: Synthetic
Genetic Insert: BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pICH47751; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson.
Primers to amplify a sgRNA:
Fw: ga GGTCTCa ATTG [x] GTTTAAGAGCTATGCTGGAA, where [x] is the 20nt spacer/target sequence (NOT including the PAM).
Rv: tgtggtctcaAGCGAAAAAAAGCACCGACTC (for polyT terminator)
tgtggtctcaAGCGACCCCAGAAATTGAAC (for 67 bp U6-26 terminator, recommanded)
tgtggtctcaAGCGTATTGGTTTATCTCATCG (for 192 bp U6-26 terminator).
The PCR amplicon is ready to clone in a Golden Gate fashion with U6-26 promoter (pICSL90002) and any Golden Gate compatible acceptor vector Level 1, using BsaI. Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117518 Copy
Species: Synthetic
Genetic Insert: BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pICH47751; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson.
Primers to amplify a sgRNA:
Fw: ga GGTCTCa ATTG [x] GTTTTAGAGCTAGAAATAGC, where [x] is the 20nt spacer/target sequence (NOT including the PAM).
Rv: tgtggtctcaAGCGAAAAAAAGCACCGACTC.
The PCR amplicon is ready to clone in a Golden Gate fashion with U6-26 promoter (pICSL90002) and any Golden Gate compatible acceptor vector Level 1, using BsaI. Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117517 Copy
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